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Expression profiles of circulating tRNA-derived small RNAs and their potential role in diabetes

by JING JIN^1,2,#, XIE LI^1,#, TING QIU^1,#, LEI SONG¹, YUANYUE CUI¹, GUANGYA ZHANG^3,4, SHU LI², WENCHENG ZHAO^5,*

1 Department of Ophthalmology, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China
2 Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China
3 Department of Endocrinology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China
4 Department of Cardiology, Shanghai Sixth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200233, China
5 Department of Medical Oncology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, China

* Corresponding Author: WENCHENG ZHAO. Email:
# Contributed equally to this work

BIOCELL 2023, 47(7), 1645-1650. https://doi.org/10.32604/biocell.2023.029493

Received 22 February 2023; Accepted 20 March 2023; Issue published 21 June 2023

Abstract

Background: This work aimed to reveal the expression profiles of tRNA-derived small RNAs (tsRNAs) in diabetes. Methods: Thirty-five diabetes patients and thirty-three controls were enrolled. The serum samples of 4 diabetes patients and 4 controls were subjected to tRF and tiRNA polymerase chain reaction (PCR) Array analysis. Then quantitative PCR (qPCR) validation was performed on all the samples. Bioinformatics analyses were conducted to explore their functions. Results: We found 115 tsRNAs that significantly differed between the two groups. 3′tiR-080-ProTGG(mt) was selected for further qPCR validation in all participants, and it was significantly decreased in diabetes patients compared with controls. Bioinformatics analysis indicated that 3′tiR-080-ProTGG(mt) may play regulatory roles via the cyclic adenosine monophosphate (cAMP) signaling pathway in the pathogenesis and progression of diabetes. Conclusion: Hence, we report that circulating tsRNAs are dysregulated that could be involved in the pathogenesis of diabetes.

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Keywords

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