Open Access

ARTICLE

RASAL2 acts as a tumor suppressor in cervical cancer cells

by LI CHEN^1,2, FANGFANG LI², SHOUYAN CAO², XIA LI², CHAO ZHOU², SAI HAN^1,*, YOUZHONG ZHANG^1,*

1 Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China
2 Department of Obstetrics and Gynecology, Binzhou Medical University Hospital, Binzhou, 256603, China

* Corresponding Authors: Sai Han, . cn; Youzhong Zhang,

BIOCELL 2023, 47(7), 1549-1560. https://doi.org/10.32604/biocell.2023.027308

Received 24 October 2022; Accepted 10 February 2023; Issue published 21 June 2023

Abstract

Background: This study was designed to investigate the roles of RASAL2 in cervical cancer (CC). Methods: Fifty-four CC tissues and 33 adjacent tissues were obtained from CC patients admitted to our hospital between March 2012 and June 2014. Real-time polymerase chain reaction and western blotting were performed to analyze the expression of RASAL2 mRNA and protein in these tissues, CC cell lines, and normal cervical cells. Over-expression and silencing of RASAL2 were induced after transfection, and the migration, invasion, and proliferation of the CC cell lines were examined. Results: RASAL2 mRNA and protein expressions were significantly down-regulated in CC tissues and cell lines than in adjacent tissues and normal cervical cells, respectively. While low RASAL2 expression correlated with advanced stage and metastasis of CC, its over-expression significantly inhibited proliferation and metastasis of CC cells and induced apoptosis. Under in vitro conditions, silencing of RASAL2 expression could significantly increase the proliferation, invasion, and migration of CC cells. Conclusion: RASAL2 functioned as a tumor suppressor in CC, and was down-regulated in CC tissue samples and cell lines. tumor suppressor in CC, and was down-regulated in CC tissue samples and cell lines.

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Keywords

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