Open Access

ARTICLE

TianmaGouteng yin attenuates ischemic stroke-induced brain injury by inhibiting the AGE/RAGE pathway

by LUOJUN ZHENG, LUAN WENG, DIWEN SHOU^*

Department of TCM Pharmacy, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310003, China

* Corresponding Author: DIWEN SHOU. Email:

(This article belongs to the Special Issue: Natural Products for Chronic Inflammatory Diseases: Pharmacology and Toxicology)

BIOCELL 2023, 47(6), 1345-1352. https://doi.org/10.32604/biocell.2023.028866

Received 13 January 2023; Accepted 16 February 2023; Issue published 19 May 2023

Abstract

Background: Ischemic stroke is characterized by permanent or transient obstruction of blood flow, leading to a growing risk factor and health burden. Tianmagouteng yin (TMG) is commonly used in Chinese medicine to treat cerebral ischemia. The aim of this study was to investigate the neuroprotective effects of TMG against ischemic stroke. Methods: Either permanent middle cerebral artery occlusion (pMCAO) or sham operation was performed on anesthetized Wistar male rats (n = 36). Results: Results demonstrated that TMG administration reduced the infarction volume and mitigated the neurobehavioral deficits. Hematoxylin and eosin (HE) staining and Prussian blue staining revealed that TMG attenuated tissue disruption and microbleeds in hippocampus tissues. In addition, TMG down-regulated the receptor of advanced glycation end products (RAGE) and p-JAK2. It also inhibited the concentrations of advanced glycation end products (AGEs), ferritin, malondialdehyde (MDA), and reactive oxygen species (ROS). Conclusion: As repetitive clinical trials of neuroprotectants targeting stroke have failed previously, our results suggested that the natural product, TMG, can probably help in the vicious cycles of ischemic stroke pathology.

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