TY - EJOU
AU - SUN, HAOTONG
AU - WANG, HEYING
AU - LI, XIN
AU - HAO, YANJIE
AU - LING, JUN
AU - WANG, HUAN
AU - WANG, FEIMIAO
AU - XU, FANG
TI - Increased MAD2L2 expression predicts poor clinical outcome in Colon Adenocarcinoma
T2 - BIOCELL
PY - 2023
VL - 47
IS - 3
SN - 1667-5746
AB - Background: Colon adenocarcinoma (COAD) is the second leading cause of cancer death worldwide thus,
identification of COAD biomarkers is critical. Mitotic Arrest Deficient 2 Like 2 (MAD2L2) is a key factor in
mammalian DNA damage repair and is highly expressed in many malignant tumors. This is a comprehensive study
of MAD2L2 expression, its diagnostic value, prognostic analysis, potential biological function, and impact on the
immune system of patients with COAD. Methods: Gene expression, clinical relevance, prognostic analysis, diagnostic
value, GO/KEGG cluster analysis, data obtained from TCGA, and bioinformatics statistical analysis were performed
using the R package. Immune responses to MAD2L2 expression in COAD were analyzed using TIMER. The
expression of MAD2L2 in HCT116 cells induced by the inflammatory factor TNF-α was detected using Western blot.
Results: Our results underscore the clinical diagnostic value and potential biological significance of MAD2L2 in
patients with COAD. A high level of MAD2L2 expression has been found in COAD and correlated with tumor status
and colon polyps. ROC curve analysis showed that MAD2L2 expression has high diagnostic value in COAD. Analysis
of immune infiltration results showed that MAD2L2 expression was positively correlated with neutrophil levels. The
western blot results demonstrated that MAD2L2 was dose-dependently present with TNF-α. GO/KEGG revealed that
MAD2L2 overexpressed and coexpressed genes were mostly involved in biological functions, including hypoxia
response, response to reduced oxygen levels, mitochondrial translation elongation, and other processes. Conclusion:
MAD2L2 as a new COAD biomarker contributes to our understanding of how alterations in gene expression and the
immunological environment contribute to the development of colon cancer. Following further investigation, MAD2L2
may prove to be a viable target factor for clinical diagnosis and therapy of COAD.
KW - MAD2L2; COAD; Poor prognosis; Immune infiltration; TCGA
DO - 10.32604/biocell.2023.026445