Open Access
ARTICLE
Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK, STAT3, and NF-κB pathways
TIANZHU LI1,#, YU ZHANG2,#, TONG ZHANG2,#, YANNAN LI2, HUI XUE2, JINGLONG CAO2, WENSHUANG HOU2, YINGHUA LUO3,*, CHENGHAO JIN2,4,*,
1 Department of Molecular Biology, College of Basic Medical Science, Chifeng University, Chifeng, 024000, China
2 Department of Biochemistry and Molecular Biology, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China
3 Department of Grass Science, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, China
4 National Coarse Cereals Engineering Research Center, Daqing, 163319, China
* Corresponding Authors: Yinghua Luo, ; Chenghao Jin,
# These authors contributed equally to this work
(This article belongs to the Special Issue: Herbal Active Ingredients: Potential for the Prevention and Treatment of Cancer)
BIOCELL 2023, 47(1), 195-204. https://doi.org/10.32604/biocell.2023.025593
Received 21 July 2022; Accepted 17 August 2022; Issue published 26 September 2022
Abstract
Schisandrin B (Sch B) is a monomer with anti-cancer and anti-inflammatory effects, which are isolated from the
plant
Schisandra chinensis (Turcz) Baillon. We investigated the anti-gastric cancer (GC) effects of Sch B and its
underlying molecular mechanisms. The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the
viability of GC and normal cell lines. Hoechst/propidium iodide staining and flow cytometry were used to assess the
apoptosis induction of Sch B. Western blotting was used to evaluate the effects of Sch B on downstream apoptotic
proteins. The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen
species (ROS) levels and related signaling pathways in GC cells. The results showed that Sch B could regulate the
phosphorylation level of mitogen-activated protein kinase (MAPK) by upregulating ROS accumulation in gastric
cancer cells, and then reduce the expression of nuclear factor kappa B (NF-κB) and phosphorylated transcription
3 (p-STAT3). In addition, Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,
and arrested cells in the G0/G1 phase. Moreover, it also inhibited cell migration, which was reversed with Nacetylcysteine pretreatment. In summary, Sch B has killing effects on GC cells by upregulating the production of
intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway, leading to the migration arrest and
apoptosis of GC cells.
Keywords
Cite This Article
APA Style
LI, T., ZHANG, Y., ZHANG, T., LI, Y., XUE, H. et al. (2023). Schisandrin B exerts anticancer effects on human gastric cancer cells through ros-mediated MAPK, STAT3, and NF-κB pathways. BIOCELL, 47(1), 195-204. https://doi.org/10.32604/biocell.2023.025593
Vancouver Style
LI T, ZHANG Y, ZHANG T, LI Y, XUE H, CAO J, et al. Schisandrin B exerts anticancer effects on human gastric cancer cells through ros-mediated MAPK, STAT3, and NF-κB pathways. BIOCELL . 2023;47(1):195-204 https://doi.org/10.32604/biocell.2023.025593
IEEE Style
T. LI et al., "Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK, STAT3, and NF-κB pathways," BIOCELL , vol. 47, no. 1, pp. 195-204. 2023. https://doi.org/10.32604/biocell.2023.025593