Open Access
ARTICLE
Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3
BINFEN HOU1, LI ZHAO1, T IANHAO ZHAO1, MINGMING YANG1, WANWAN ZHU1, XIAODONG CHEN2, XIQUAN KE1, ZHENZENG MA1, LIN GU1, MENG WANG1, MIN DENG1,*
1
Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, China
2
Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, China
* Corresponding Author: Min Deng,
BIOCELL 2023, 47(1), 175-186. https://doi.org/10.32604/biocell.2022.021359
Received 10 January 2022; Accepted 24 May 2022; Issue published 26 September 2022
Abstract
Aim: Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported to
possess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigate
the effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells.
Methods: Human GC
cell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation,
migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwell
assay, and flow cytometry. Cell migration and invasion were reassessed in these transfected cells following the
transfection of nod-like receptor protein-3 (NLRP3) siRNA in MKN 28 and AGS cells. To examine the downstream
signaling pathway of the NLRP3 signaling pathway, NLRP3, caspase-1, gasdermin-D, interleukin (IL)-1β, and IL-18
were detected by quantitative real-time-polymerase chain reaction or western blotting.
Results: Chrysophanol
inhibited the proliferation of GC cells, caused pyroptosis, inhibited cell migration and invasion, and increased the
expression of NLRP3 inflammasomes in GC cells. Knockdown of NLRP3 inhibited the effects of chrysophanol on
proliferation, pyroptosis, migration, and invasion of GC cells. Chrysophanol plays an anticancer role by enhancing
NLRP3.
Conclusions: Chrysophanol exerts anti-neoplastic effects in vitro in GC cells by modulating NLRP3, thus
highlighting its therapeutic potential in GC.
Keywords
Cite This Article
APA Style
HOU, B., ZHAO, L., ZHAO, T.I., YANG, M., ZHU, W. et al. (2023). Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3. BIOCELL, 47(1), 175-186. https://doi.org/10.32604/biocell.2022.021359
Vancouver Style
HOU B, ZHAO L, ZHAO TI, YANG M, ZHU W, CHEN X, et al. Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3. BIOCELL . 2023;47(1):175-186 https://doi.org/10.32604/biocell.2022.021359
IEEE Style
B. HOU et al., "Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3," BIOCELL , vol. 47, no. 1, pp. 175-186. 2023. https://doi.org/10.32604/biocell.2022.021359