Open AccessOpen Access


The expression and function of miR-376a-3p/DLX axis in gastric cancer cells


Department of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, 100853, China

* Corresponding Authors: CHAOJUN ZHANG. Email: ; LIN CHEN. Email:

BIOCELL 2022, 46(9), 2073-2080.


Gastric cancer (GC) was referred to a malignant tumor of the digestive tract originating from the epithelium of gastric mucosa. Transcription factor DLX5 was verified as an oncogene in various types of tumors, while miR-376a-3p was speculated as a tumor suppressor. Based on the bioinformatics database, we hypothesized that miR-376a participated in the regulation of GC development by targeting DLX5. Compared with adjacent tissue, a significant increase of DLX5 expression was determined in GC tissues, but the expression level is significantly reduced in miR-376a. Similar expression signature of DLX5 and miR-376a was also determined between 4 GC cells (HGC, SGC, MGC, and AGS cell lines) and GES cell line. The level of DLX5 was notably reduced in HGC and MGC cell lines after miR-376a-3p overexpression, and increased after miR-376a-3p inhibition. Then, the inhibition role of miR-376a-3p on DLX5 was further proved by dual-luciferase reporter assay. Gain-of-function experiments showed that upregulation of miR-376a-3p in GC cells could inhibit the ability of epithelial-mesenchymal transition, proliferation, and invasion, and enhance the GC cell apoptosis level. However, these roles of miR-376a-3p could be abolished by DLX5 overexpression. This study confirmed that reduction of miR-376a-3p expression level in GC cells would lead to the increase in cell growth and invasion, indicating that upregulation of miR-376a-3p might have a potential therapeutic role on GC.


Cite This Article

ZHANG, Y., ZHANG, C., CAO, Z., ZHAO, Z., CHEN, L. (2022). The expression and function of miR-376a-3p/DLX axis in gastric cancer cells. BIOCELL, 46(9), 2073–2080.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 537


  • 214


  • 0


Share Link

WeChat scan