Open Access
ARTICLE
Transcriptome analysis combined with metabolome analysis reveals the significant functions of CesA genes in cotton (Gossypium hirsutum) fiber length development
ZHENKUI CUI1,#,*, GUIQIN SUN2,#,*, QUANZHI ZHAO1
1 College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China
2 Jiangxi Agricultural Engineering Vocational College, Zhangshu, 331200, China
* Corresponding Authors: ZHENKUI CUI. Email: ; GUIQIN SUN. Email:
BIOCELL 2022, 46(9), 2133-2144. https://doi.org/10.32604/biocell.2022.020512
Received 28 November 2021; Accepted 17 January 2022; Issue published 18 May 2022
Abstract
Cotton is widely distributed worldwide, and improving the quality of its fiber is one of the most important tasks in cotton breeding. Cotton fibers are primarily composed of cellulose, which is synthesized by
CesA complexes (CSCs). However, the functions of
CesA genes in cotton fiber development have not been comprehensively analysed. In this study, the cotton transcriptome and metabolome were used to investigate the function o
f CesA genes in fiber development. Finally, 321 metabolites were obtained, 84 of which were associated with the corresponding genes. Interestingly, a target gene named
Gh_A08G144300, one of the
CesA gene family members, was closely correlated with the development of cotton fibers. The target
CesA gene
Gh_A08G144300 was analysed to determine its specific function in cotton fiber development. High-level gene expression of
Gh_A08G144300 was found at different fiber development stages by RNA-seq analysis, and the silencing of
Gh_A08G144300 visibly inhibited the growth of cotton fibers, showing that it is critical for their growth. This study provides an important reference for research on the gene function of
Gh_A08G144300 and the regulatory mechanism of fiber development in cotton.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
CUI, Z., SUN, G., ZHAO, Q. (2022). Transcriptome analysis combined with metabolome analysis reveals the significant functions of
CesA genes in cotton (
Gossypium hirsutum) fiber length development.
BIOCELL, 46(9), 2133–2144. https://doi.org/10.32604/biocell.2022.020512