Open Access
ARTICLE
Effect of Lycopus lucidus Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice
EUN NA1, SUN YOUNG LIM2,*
1 Ocean Science and Technology School, Korea Maritime & Ocean University, Busan, Korea
2 Division of Convergence on Marine Science, Korea Maritime & Ocean University, Busan, Korea
* Corresponding Author: Sun Young Lim,
BIOCELL 2022, 46(9), 2101-2109. https://doi.org/10.32604/biocell.2022.019807
Received 16 October 2021; Accepted 31 December 2021; Issue published 18 May 2022
Abstract
We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice. In
addition, we evaluated the production of major cytokines (Interleukin-6 and -10) which are related to inflammation
and fatty acid composition of several tissues. 16S ribosomal DNA sequencing-based microbiome taxonomic profiling
analysis was performed utilizing the EzBioCloud data base. Male mice fed on
L. lucidus showed a significantly
reduced number of lactic acid bacteria and coliform in the feces compared with the control group (
p < 0.05). 16S
rDNA sequencing analysis of fecal samples showed that
L. lucidus supplementation decreased the community of
harmful microflora (
Enterobacteriaceae including
Escherichia coli and
Bacteroides sp.) in feces compared with the
control group (p < 0.05). There were no significant differences in mRNA expression of cytokine IL-6 and IL-10
between the control and
L. lucidus fed groups. The fecal fatty acid composition in the
L. lucidus group had
percentages of 4:0, 6:0, 8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control
group. Our results showed that
L. lucidus supplementation influenced gut environment by decreasing harmful
microflora and increased the percentages of several short fatty acids.
Keywords
Cite This Article
NA, E., LIM, S. Y. (2022). Effect of
Lycopus lucidus Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice.
BIOCELL, 46(9), 2101–2109.