TY - EJOU
AU - CAO, MING-AO
AU - ZHANG, FEI
AU - ABD_ALLAH, ELSAYED FATHI
AU - WU, QIANGSHENG
TI - Mycorrhiza improves cold tolerance of Satsuma orange by inducing antioxidant enzyme gene expression
T2 - BIOCELL
PY - 2022
VL - 46
IS - 8
SN - 1667-5746
AB - A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (Diversispora
versiformis) and arbuscular mycorrhizal like fungus (Piriformospora indica) on antioxidant enzyme defense system of
Satsuma orange (Citrus sinensis cv. Oita 4) grafted on Poncirus trifoliata under favourable temperature (25°C) and
cold temperature (0°C) for 12 h. Such short-term treatment of cold temperature did not cause any significant change
in root fungal colonization and spore density in soil. Under cold stress, D. versiformis inoculation did not change the
activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots, whereas P. indica
inoculation significantly increased the activity of CAT in roots and POD in leaves only. In addition, inoculation of
two mycorrhizal fungi under cold stress significantly increased the relative expression levels of PtPOD and PtF-SOD
in leaves, P. indica up-regulated the expression levels of PtCu/Zn-SOD in leaves, and D. versiformis also induced the
expression levels of PtMn-SOD and PtCAT1 in leaves. In addition, inoculated Oita 4 trees maintained significantly
lower hydrogen peroxide levels and malondialdehyde contents in leaves and roots under cold temperature, suggesting
lower oxidative damage. Therefore, we concluded that arbuscular mycorrhizal fungi (especially P. indica) mainly
induced the expression of antioxidant enzyme genes, depending on the fungal species, and thus mitigated oxidative
damage for higher cold resistance in inoculated plants.
KW - Antioxidant defense system
KW - Citrus
KW - Cold stress
KW - Mycorrhiza
KW - Oxidative burst
DO - 10.32604/biocell.2022.020391