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ARTICLE
Mycorrhiza improves cold tolerance of Satsuma orange by inducing antioxidant enzyme gene expression
MING-AO CAO1, FEI ZHANG2, ELSAYED FATHI ABD_ALLAH3, QIANGSHENG WU1,*
1 College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
2 College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, 438000, China
3 Plant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia
* Corresponding Author: Qiangsheng Wu,
(This article belongs to this Special Issue: Mycorrhizal Fungal Roles in Stress Tolerance of Plants)
BIOCELL 2022, 46(8), 1959-1966. https://doi.org/10.32604/biocell.2022.020391
Received 21 November 2021; Accepted 06 January 2022; Issue published 22 April 2022
Abstract
A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (
Diversispora
versiformis) and arbuscular mycorrhizal like fungus (
Piriformospora indica) on antioxidant enzyme defense system of
Satsuma orange (
Citrus sinensis cv. Oita 4) grafted on Poncirus trifoliata under favourable temperature (25°C) and
cold temperature (0°C) for 12 h. Such short-term treatment of cold temperature did not cause any significant change
in root fungal colonization and spore density in soil. Under cold stress,
D. versiformis inoculation did not change the
activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots, whereas P. indica
inoculation significantly increased the activity of CAT in roots and POD in leaves only. In addition, inoculation of
two mycorrhizal fungi under cold stress significantly increased the relative expression levels of
PtPOD and
PtF-SOD
in leaves,
P. indica up-regulated the expression levels of
PtCu/Zn-SOD in leaves, and
D. versiformis also induced the
expression levels of
PtMn-SOD and
PtCAT1 in leaves. In addition, inoculated Oita 4 trees maintained significantly
lower hydrogen peroxide levels and malondialdehyde contents in leaves and roots under cold temperature, suggesting
lower oxidative damage. Therefore, we concluded that arbuscular mycorrhizal fungi (especially
P. indica) mainly
induced the expression of antioxidant enzyme genes, depending on the fungal species, and thus mitigated oxidative
damage for higher cold resistance in inoculated plants.
Keywords
Cite This Article
CAO, M., ZHANG, F., ABD_ALLAH, E. F., WU, Q. (2022). Mycorrhiza improves cold tolerance of Satsuma orange by inducing antioxidant enzyme gene expression.
BIOCELL, 46(8), 1959–1966.