Open Access

ARTICLE

Genome-wide analysis of microRNAs induced and regulated by colchicine in mulberry

MENGMENG WU^1,#, PENG GUO^1,#, YISU SHI^1,#, DANYAN ZHENG¹, QIAONAN ZHANG¹, XIN JIN¹, YUHUA WANG¹, QIANG LIN², RONGJUN FANG^1,*, WEIGUO ZHAO^1,*

1 Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, School of Biology and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China
2 Sericultural Research Institute, Nanning, 530007, China

* Corresponding Authors: Rongjun Fang, ; Weiguo Zhao,
# These authors contributed equally to this work

(This article belongs to the Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)

BIOCELL 2022, 46(8), 1935-1945. https://doi.org/10.32604/biocell.2022.019223

Received 10 September 2021; Accepted 25 November 2021; Issue published 22 April 2022

Abstract

A variety of plants were colchicine treated to double their chromosome number. Chromosomes are genetic carriers that determine the individual traits of organisms. The doubling of chromosomes will lead to modifications in plant morphology, physiology and genetics. To determine the response of mulberry trees induced by colchicine, using mulberry variety Yu-711 leaves as research materials, two small RNA libraries (control and experimental groups) were constructed. It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results. A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA genes, 19 genes of which were up-regulated and 18 genes of which were down-regulated. Eight miRNAs were selected from 37 differentially expressed miRNA genes. These miRNAs were verified by quantitative real-time PCR, and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing, which proved the accuracy of sequencing results. The PsRNATarget software was used to predict the target genes of 8 miRNAs. The Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect the functions of target genes and confirm that target genes are mainly involved in biological processes, cell components and molecular functions. novel-77 miRNA was selected from 37 differentially expressed miRNAs. What will serve as a fundamental data in understanding mulberry miRNAs function in molecular biology research and further provides the molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77 precursor sequence and the target gene (XM_010104258.2), as well as the secondary structure analysis.

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Keywords

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