Open Access
VIEWPOINT
Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development
SITI NABILAH1,2; TRI HANDOYO2,3; KYUNG-MIN KIM4; MOHAMMAD UBAIDILLAH2,*
1 Graduate School of Biotechnology, University of Jember, Jember, 68121, Indonesia
2 Faculty of Agriculture, University of Jember, Jember, 68121, Indonesia
3 Center Development of Advanced Science and Technology (CDAST), University of Jember, Jember, 68121, Indonesia
4 Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, 41566,
South Korea
* Corresponding Author: Mohammad Ubaidillah,
(This article belongs to this Special Issue: Tumor Microenvironment and Cytoskeletal Dynamics)
BIOCELL 2022, 46(7), 1633-1641. https://doi.org/ 10.32604/biocell.2022.019111
Received 03 September 2021; Accepted 28 October 2021; Issue published 17 March 2022
Abstract
Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice.
This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy
Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development
and clones and transformations. Here, we report the gene expression during somatic embryo development correlates
with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media
supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding
the genetic basis of somatic embryogenesis in other varieties, rice is still unexplored, especially during somatic embryo
development. Moreover, for the formation of callus induction from immature embryos, 2,4-D (2.0 mg/L, 3.0 mg/L)
was used. This study analysed the gene expression of
OsSERK,
OsWOX4 and
OsLEC1 genes through RT-PCR analysis.
Higher expression of the
OsLEC1 gene indicates that their function may correlate in the in vitro with the high
response of rice after transfer to regeneration media. This study found that rice varieties of pigmented rice (MS
Pendek and Gogoniti II) and non-pigmented rice (Pandan Ungu) showed high regeneration frequency, showing
higher
OsLEC1 expression than other varieties because
OsLEC1 promotes the maturation of somatic embryos in plant
regeneration on day 14. However, the contrast with Genjah nganjuk may be effective because of other regulatory
genes. RT-PCR analysis showed
OsSERK had less expression level than
OsLEC1 and
OsWOX4 in the varieties, which
correlate with the percentage of plant regeneration, but not for Gogoniti II. In conclusion, the higher percentage of
plant regeneration correlates with the higher expression level of
OsLEC1 at day 14 of media regeneration of rice.
Keywords
Cite This Article
NABILAH, S. (2022). Expression analysis of
OsSERK,
OsLEC1 and
OsWOX4 genes in rice (
Oryza sativa L.) callus during somatic embryo development.
BIOCELL, 46(7), 1633–1641. https://doi.org/ 10.32604/biocell.2022.019111