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The molecular characteristics of soybean ARR-B transcription factors

HE LI1, RUNAN CHEN1, ZHONGCHENG CHEN1, JIAXIN LIN1, XIJUN JIN1, CHUNYUAN REN1, QIUSEN CHEN1, FENGQIONG CHEN1, GAOBO YU1,*, YUXIAN ZHANG1,2,*

1 Heilongjiang Bayi Agricultural University, Daqing, 163319, China
2 National Coarse Cereals Engineering Technology Research Center, Daqing, 163319, China

* Address correspondence to: Gaobo Yu, email; Yuxian Zhang, email

BIOCELL 2022, 46(6), 1575-1592. https://doi.org/10.32604/biocell.2022.018762

Abstract

The Type-B authentic response regulator (ARR-Bs) gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes. However, study of ARR-Bs gene family in soybean is limited. Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome. 31 ARR-Bs genes (namely GmARR-B1-31) were identified, containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids (aa) and 28.30 to 76.86 kDa, respectively. Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I, Cluster II, and Cluster III—which included 15, 12, and 4 genes, respectively, and were asymmetrically distributed on 17 chromosomes. Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers, roots and seeds. The subcellular localization of GmARR5, GmARR14 were observed in the nucleus, and the promoter region of them included low-temperature responsive element (LTR), the C-repeat/dehydration responsive element (DRE), MBS, ABRE, MYB, MEJA and TCA-elements, which possibly participate in abiotic stress and hormones responses. qRT-PCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses, especially cold stress and salt stress, and GmARR-B5 and GmARR-B14 were significantly induced by cold stress. This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.

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LI, H., CHEN, R., CHEN, Z., LIN, J., JIN, X. et al. (2022). The molecular characteristics of soybean ARR-B transcription factors. BIOCELL, 46(6), 1575–1592.



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