Open Access

ARTICLE

Effect of VirD4 on gastric epithelial-1 cells and its mechanism

YANG YANG^1,*, BENSHUAI YOU², CHENGLIN ZHOU^1,*

1 Clinical Laboratory Center, Jiangsu Taizhou People’s Hospital, Taizhou, 225399, China
2 School of Medicine, Jiangsu University, Zhenjiang, 212013, China

* Address correspondence to: Yang Yang, ; Chenglin Zhou,

BIOCELL 2022, 46(6), 1557-1564. https://doi.org/10.32604/biocell.2022.018326

Received 16 July 2021; Accepted 16 September 2021; Issue published 07 February 2022

Abstract

The gene of Helicobacter pylori can encode three to four type IV secretory systems, of which a new gene region has been found in the H. pylori plasticity region. The coding products of this region can form a new T4SS named tfs3, but its function is unclear. This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H. pylori clinical strain SBK on GES-1 cells. We observed changes in cell morphology after VirD4 treatment. Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB. VirD4 can also inhibited proliferation, and induced migration of cells. Moreover, VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events. Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H. pylori-related diseases.

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