Open Access

ARTICLE

Effect of RBM10 on regulating the proliferation and metastasis activity of human hepatocellular carcinoma cells by affecting the stability of miR-21

LI JIANG^1,*, KE ZHANG¹, CHONG HU¹, HONGWEI ZHANG¹, DANPU WANG¹, WEIWEI ZHAI²

1 Department of General Surgery, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China
2 Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

* Corresponding Author: LI JIANG. Email:

(This article belongs to the Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)

BIOCELL 2022, 46(4), 969-978. https://doi.org/10.32604/biocell.2022.017765

Received 04 June 2021; Accepted 08 July 2021; Issue published 15 December 2021

Abstract

In recent decades, RNA binding motif (RBM) proteins have been widespread concerned by researchers. Among them, RBM5 is considered as a potential tumor suppressor gene in HCC. RBM10, also belonging to the RBM family, have similar structure and high homology with RBM5, indicating its potential as potential tumor suppressor genes. However, the role of RBM10 in tumors is controversial. The purpose of this study was to analyze the expression correlation and functional relationship of miR-21 and RBM10 in human hepatocellular carcinoma (HCC) tissues and corresponding tumor cells. Bioinformatics analysis showed that miR-21 and RBM10 were both highly expressed in HCC; similarly, the expression levels of miR-21 and RBM10 in HCC cells were significantly higher than those in normal hepatocytes. There was a positive correlation between miR-21 and RBM10. Furthermore, knockdown of RBM10 inhibited the proliferation, migration and invasion of SNU-398 cells, and simultaneous overexpression of miR-21 attenuated this inhibitory effect. Meanwhile, overexpressing RBM10 could promote the proliferation, migration and invasion of Hep G2 cells; while the stability of miR-21 could be reduced by knocking down RBM10. On the whole, the findings of this study indicate that RBM10 is involved in regulating the function and activity of human HCC cells by affecting the stability of miR-21. RBM10 cannot be simply summarized as a tumor suppressor or oncogene. Further studies are needed to clarify the role of RBM10 in tumors.

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