HAND2-AS1, PRKAA2 and VLDLR predict the risk of peritoneal metastasis in gastric cancer of different Lauren types based on STEPP analysis
HUI QU1,2,3,4,#, RUICHUAN SHI1,2,3,4,#, BOWEN YANG1,2,3,4, XI CHEN1,2,3,4, YUJIA SONG1,2,3,4, LINGZI HE1,2,3,4, YITING SUN1,2,3,4, YUJING YANG1,2,3,4, ZHI LI1,2,3,4,*, XIUJUAN QU1,2,3,4,*
1 Department of Medical Oncology, The First Hospital of China Medical University, Shenyang, 110001, China
2 Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province, The First Hospital of China Medical University, Shenyang, 110001, China
3 Liaoning Province Clinical Research Center for Cancer, Shenyang, 110001, China
4 Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, Shenyang, 110001, China
# These authors contributed equally to this work
* Corresponding Authors:XIUJUAN QU. Email: ; ZHI LI. Email:
BIOCELL 2022, 46(3), 721-735. https://doi.org/10.32604/biocell.2021.015867
Received 21 January 2021; Accepted 31 March 2021; Issue published 18 November 2021
Abstract
The peritoneum is the most common site of recurrence of gastric cancer (GC). Early occult peritoneal
metastasis is difficult to detect by imaging examination. Stratifying the risk of peritoneal metastasis in patients with
different Lauren subtypes is of great clinical value. We performed a univariate Cox regression to identify those genes
with prognostic value of overall survival (OS) and peritoneal-specified disease-free survival (psDFS) from the Gene
Expression Omnibus database. The candidate genes were screened by the Subpopulation Treatment Effect Pattern
Plot (STEPP) method. Propensity score matching (PSM) analysis was used to reduce the interference of confounders
on the results. Based on the optimal cut-off values determined by the STEPP method, we found overexpression of
three genes (HAND2-AS1, PRKAA2, and VLDLR) was correlated with shorter 1-year psDFS among patients with
diffuse-type than that of patients with intestinal-type GC, and it is highly significant. Gene Set Enrichment Analysis
(GSEA) potentially suggested that the three genes promote the early occurrence of peritoneal metastasis in patients
with diffuse-type GC through glucose metabolism-related pathways. These three genes may be potential biomarkers.
They can be used to assess the risk of peritoneal metastases to guide treatment decisions and follow-up strategies.
Keywords
Cite This Article
Qu, H., Shi, R., Yang, B., Chen, X., Song, Y. et al. (2022). HAND2-AS1, PRKAA2 and VLDLR predict the risk of peritoneal metastasis in gastric cancer of different Lauren types based on STEPP analysis.
BIOCELL, 46(3), 721–735.