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The characterization of transmembrane protein 59-like (TMEM59L) reveals its role in the regulating the level of the GDI protein family


1 School of Environmental Engineering, Central Plains Specialty Food Engineering & Technology Research Center, Kaifeng Key Laboratory of Food Composition and Quality Assessment, Yellow River Conservancy Technical Institute, Kaifeng, 475004, China
2 Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, Shanghai, 200444, China

* Corresponding Authors: HAIFENG WANG. Email: ; TIEQIAO WEN. Email:

BIOCELL 2022, 46(12), 2615-2624.


The characterization and functions of transmembrane protein 59-like (TMEM59L), a type I transmembrane protein, are not clearly understood until now. Some TMEM59L and fluorescent fusion proteins constructs were transfected in cell lines and liposomes, and their localization was observed. The effects of protein constructs were studied by fluorescence microscopy and western blotting. This study reports a novel function of human TMEM59L (hTMEM59L) related to the expression and location of some proteins. In addition, we report two novel splice variants of human TMEM59L (hTMEM59L). The localization of mutants of this protein, lacking a middle region, and a C-terminal deletion, markedly differed from that of full-length hTMEM59L. Intracellular movement assessment in living cells showed the localization of TMEM59L to vesicular structures in the Golgi bodies, and the cell membrane was observed in living cells. Overexpression of TMEM59L markedly increased the level of amyloid precursor protein because of TMEM59L-mediated inhibition of cell membrane transport but did not affect the expression of beta-secretase 2. TMEM59L overexpression also significantly increased the levels of Rab GDP dissociation inhibitor alpha and Rab GDP dissociation inhibitor beta proteins. These results suggest that TMEM59L is involved in the packaging of acidic vesicles and thereby functions in the trafficking and processing of intracellular proteins.


Cite This Article

WANG, H., GUO, J., WEN, T. (2022). The characterization of transmembrane protein 59-like (TMEM59L) reveals its role in the regulating the level of the GDI protein family. BIOCELL, 46(12), 2615–2624.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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