TY - EJOU AU - LIU, HUOGEN AU - SHI, YUNDI AU - WAN, XIN AU - LIU, YING AU - SHU, HAILIN AU - HUANG, FENGMING AU - GONG, ZHENBIN AU - GU, LING TI - Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition T2 - BIOCELL PY - 2022 VL - 46 IS - 12 SN - 1667-5746 AB - Peroxiredoxin 1 (PRDX1) participates in tumor cell proliferation, apoptosis, migration, invasion, and the epithelial-to-mesenchymal transition (EMT). This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide (LPS) and transforming growth factor-beta 1 (TGF-β1). PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial (BEAS-2B) cells, reduced cell apoptosis (p < 0.01), and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase (MMP)2, MMP9, α-smooth muscle actin (α-SMA), N-cadherin, vimentin and twist proteins and inhibiting E-cadherin expression (p < 0.05). PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis (p < 0.05). Knockdown of PRDX1 inhibited cell proliferation, migration, EMT, and collagen synthesis (p < 0.01), reversed LPS-mediated inhibition of cell proliferation and migration, and significantly suppressed LPS-induced EMT and collagen synthesis (p < 0.01). The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells. KW - Peroxiredoxin 1; Airway epithelial cell; Epithelial-to-mesenchymal transition; Cell migration; Collagen synthesis DO - 10.32604/biocell.2022.018054