Open Access iconOpen Access

ARTICLE

Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition

HUOGEN LIU#, YUNDI SHI#, XIN WAN, YING LIU, HAILIN SHU, FENGMING HUANG, ZHENBIN GONG, LING GU*

Department of Critical Care Medicine, Mindong Hospital Affiliated to Fujian Medical University, Ningde, 355000, China

* Corresponding Author: LING GU. Email: email

(This article belongs to the Special Issue: Single Cell Technologies and Molecular Mechanisms of Diseases)

BIOCELL 2022, 46(12), 2671-2680. https://doi.org/10.32604/biocell.2022.018054

Abstract

Peroxiredoxin 1 (PRDX1) participates in tumor cell proliferation, apoptosis, migration, invasion, and the epithelial-to-mesenchymal transition (EMT). This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide (LPS) and transforming growth factor-beta 1 (TGF-β1). PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial (BEAS-2B) cells, reduced cell apoptosis (p < 0.01), and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase (MMP)2, MMP9, α-smooth muscle actin (α-SMA), N-cadherin, vimentin and twist proteins and inhibiting E-cadherin expression (p < 0.05). PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis (p < 0.05). Knockdown of PRDX1 inhibited cell proliferation, migration, EMT, and collagen synthesis (p < 0.01), reversed LPS-mediated inhibition of cell proliferation and migration, and significantly suppressed LPS-induced EMT and collagen synthesis (p < 0.01). The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.

Keywords


Cite This Article

APA Style
LIU, H., SHI, Y., WAN, X., LIU, Y., SHU, H. et al. (2022). Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. BIOCELL, 46(12), 2671-2680. https://doi.org/10.32604/biocell.2022.018054
Vancouver Style
LIU H, SHI Y, WAN X, LIU Y, SHU H, HUANG F, et al. Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. BIOCELL . 2022;46(12):2671-2680 https://doi.org/10.32604/biocell.2022.018054
IEEE Style
H. LIU et al., “Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition,” BIOCELL , vol. 46, no. 12, pp. 2671-2680, 2022. https://doi.org/10.32604/biocell.2022.018054



cc Copyright © 2022 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 951

    View

  • 629

    Download

  • 0

    Like

Share Link