Open Access

ARTICLE

Complete genome sequence of two strawberry vein banding virus isolates from China

LEI JIANG^1,2,3,#, SHUAI LI^1,#, XIZI JIANG¹, TONG JIANG^1,2,3,*

1 School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China
2 Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei, 230036, China
3 Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei, 230036, China

* Corresponding Author: Tong Jiang,
# These authors contributed equally to this paper

BIOCELL 2021, 45(6), 1695-1702. https://doi.org/10.32604/biocell.2021.015250

Received 04 December 2020; Accepted 08 February 2021; Issue published 01 September 2021

Abstract

It was rarely reported about strawberry vein banding virus (SVBV) genome sequence in China and most countries worldwide. In this work, we determined the complete genome sequences of two SVBV isolates in China, designated SVBV-AH and SVBV-BJ, that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China, respectively. The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides (nts) and 7,863 nts long, respectively, and both constituted with seven genes typical of the caulimoviruses. Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7% of each other and had 85.7% and 86.0% sequence identity related to SVBV from the United States (SVBV-US), respectively. Phylogenetic trees, based on the alignment of complete nucleotide sequences and amino acid sequences of Coat Protein (CP), both showed that SVBV-AH and SVBV-BJ clustered into one branch with all the other SVBV isolates, and other species of caulimoviruses clustered into another tree branch. It illustrated that all the SVBV isolates had an extremely high relationship but had a distant relationship with other species of caulimoviruses. We further confirmed that SVBV-AH infectious clone could cause similar symptoms to SVBVinfected in strawberry under natural conditions. Taken together, our study provided valuable information to elucidate the origin and dissemination of SVBV Chinese isolates, meanwhile providing the necessary vector for studying the gene functions of strawberry.

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