Open Access

ARTICLE

High level of circPTN promotes proliferation and stemness in gastric cancer

KEWEI GAO^1,#, JIANGFENG HU^2,#, YI ZHOU³, LIANG ZHU^1,*

1 Department of Gastroenterology, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, 200001, China
2 Department of Gastroenterology, Shanghai First People’s Hospital, Shanghai, 200003, China
3 Department of Gastroenterology, Shanghai Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 200003, China

* Corresponding Author: LIANG ZHU. Email:

BIOCELL 2021, 45(6), 1521-1526. https://doi.org/10.32604/biocell.2021.09220

Received 26 November 2019; Accepted 14 April 2020; Issue published 01 September 2021

Abstract

Increasing evidence proves that circular RNAs (circRNAs) play an important role in regulating the biological behaviors of tumors. The central purpose of this research was to investigate the functions of circRNA in gastric cancer. The utilization of real-time PCR was to test circPTN expression in gastric cancer cells. Cell counting colony formation assays, CCK-8 assay, and EdU assay were used to investigate proliferation. Transwell assay was applied to investigate migration. We discovered that circPTN was highly expressed in gastric cancer cells. Low expression of circPTN inhibits gastric cancer cell proliferation and migration. Elevated expression of circPTN promotes gastric cancer cell proliferation and migration. Moreover, we discovered that circPTN could accelerate self-renewal and increase the expression of stemness markers. The results of our study suggested that a high level of circPTN expression promotes the proliferation and stemness of gastric cancer cells.

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Keywords

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