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Real-Time analysis of exosome secretion of single cells with single molecule imaging

PENGFEI ZHANG¹, SHAOPENG WANG^1,2,*

1 Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, 85287, USA
2 School of Biological and Health Systems Engineering, Arizona State University, Tempe, 85287, USA

* Corresponding Author: SHAOPENG WANG. Email:

(This article belongs to the Special Issue: Single Cell Analysis: Technology and Application)

BIOCELL 2021, 45(6), 1449-1451. https://doi.org/10.32604/biocell.2021.017607

Received 24 May 2021; Accepted 01 July 2021; Issue published 01 September 2021

Abstract

The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity.

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