Open Access

ARTICLE

Identification of PtGai (a DELLA protein) in trifoliate orange and expression patterns in response to drought stress

XIAOFEN CHENG¹, ABEER HASHEM^2,3, ELSAYED FATHI ABD_ALLAH⁴, QIANGSHENG WU^1,5,*, KAMIL KUČA^5,*

1 College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
2 Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
3 Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza, 12511, Egypt
4 Plant Production Department, Faculty of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia
5 Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, 50003, Czech Republic

* Corresponding Authors: QIANGSHENG WU. Email: ; KAMIL KUČA. Email:

BIOCELL 2021, 45(6), 1687-1694. https://doi.org/10.32604/biocell.2021.017581

Received 21 May 2021; Accepted 29 June 2021; Issue published 01 September 2021

Abstract

Gibberellins (GAs) are an important hormone in regulating plant growth and development, and DELLA protein is an essential negative regulator of GA signal transduction. The aim of the study was to clone a GA-inhibiting protein DELLA from trifoliate orange (Poncirus trifoliata L. Raf.) and to analyze the bioinformations and expression patterns of the protein gene in tissues and in response to drought stress. A DELLA protein was isolated from trifoliate orange and named as PtGai (Genebank number: MZ170959). The PtGai protein had 1731 bp open reading frames, along with 576 amino acid codes, and also grouped with sweet orange (XM_006430552.4). The PtGai protein sequence was 65% homology with the sequences of DELLA proteins in other plant families. PtGai protein existed in the nucleus based on the prediction of subcellular localization. PtGai protein could be expressed in roots, stems, and leaves, along with the highest expression in stems. PtGai was upregulated by drought stress in leaves and roots, along with the decrease of root total GA concentration and the inhibition of shoot and root biomass production. It indicated the characteristics of PtGai protein and the roles of PtGai in GA synthesis and plant growth.

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Keywords

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