Open Access

ARTICLE

Investigation of the antioxidant defensive role of both AD-MSCs and BM-MSCs in modulating the alteration in the oxidative stress status in various STZ-diabetic rats’ tissues

by SHADY G. EL-SAWAH^1,*, FAYEZ ALTHOBAITI², ADIL ALDHAHRANI³, EMAN FAYAD², MARWA A. ABDEL-DAYEM⁴, REHAB M. AMEN⁵, EL SHAIMAA SHABANA⁶, EHAB I. EL-HALLOUS⁷, HANAN M. RASHWAN¹

1 Zoology Department, Faculty of Science, Arish University, North Sinai, Egypt
2 Biotechnology Department, Faculty of Science, Taif University, Taif, 21944, Saudi Arabia
3 Clinical Laboratory Science Department, Turabah University College, Taif University, Taif, 21995, Saudi Arabia
4 Department of Pharmacology and Biochemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta, 34518, Egypt
5 Biology Department, College of Science, University of Bisha, Bisha, 61922, Saudi Arabia
6 Fellow of Biochemistry, Genetic Unit, Children Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt
7 Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia

* Corresponding Author: SHADY G. EL-SAWAH. Email:

(This article belongs to the Special Issue: Cell-Based Regenerative Therapies)

BIOCELL 2021, 45(6), 1561-1568. https://doi.org/10.32604/biocell.2021.016869

Received 04 April 2021; Accepted 14 May 2021; Issue published 01 September 2021

Abstract

Diabetes mellitus (DM) could negatively affect patients’ health via inducing a lot of serious functional hazards in many tissues’ cells at molecular levels. Recently, many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes. Therefore, the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’ oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction. In our 4 weeks study, animals were divided into four groups: control group, STZ-diabetic group (D), D+AD-MSCs group and D+BM-MSCs group. Data reported that diabetic rats treated with either AD-MSCs or BM-MSCs exhibited a marvelous body tissues (Pancreas, Liver and Kidney) enhancing capabilities in attenuating the oxidative stress status; as evidenced by XO, ROS, and MDA levels down-regulation; with a general concomitant elevation in the antioxidants’ content; evidenced by many enzymatic and non-enzymatic antioxidants up-regulation; relative to the diabetic untreated group. Interestingly, comparing both treatments with each other and to control group, most of the measured parameters were reverted back to near normal levels after AD-MSCs injection; which clearly point out their stunning health benefits and superiority as anti-diabetic agent in overcoming different tissues’ complications; owing to their marked cytoprotective and regenerative potentialities.

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Keywords

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