Open Access

ARTICLE

Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale × wheat derived doubled haploid lines

by MUNIYANDI SAMUEL JEBERSON^1,*, HARINDER KUMAR CHAUDHARY², RAKESH KUMAR CHAHOTA³, SHABIR HUSSAIN WANI^4,*

1 AICRP(MULLaRP), Directorate of Research, CAU, Imphal, India
2 Molecular Cytogenetics and Tissue Culture Laboratory, CSKHPKV, Palampur, India
3 Department of Agricultural Biotechnology, CSKHPKV, Palampur, India
4 Mountain Research Center for Field Crops, Sher-e-Kashmir University of Agricultural Sciences and Technology, Khudwani, India

* Corresponding Authors: MUNIYANDI SAMUEL JEBERSON. Email: ; SHABIR HUSSAIN WANI. Email:

BIOCELL 2021, 45(6), 1651-1659. https://doi.org/10.32604/biocell.2021.015735

Received 09 January 2021; Accepted 24 May 2021; Issue published 01 September 2021

Abstract

The rye genome has shown potential for improvement of bread wheat, where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding. Crosses belongs to different generations viz., BC₁F₁, BC₁F₂, BC₁F₃, BC₁F₄ and BC₂F₃ of triticale × wheat derived were used for different haploid induction parameters using Gogon grass (Imperata cylindrica) as a pollen source. The percentage of pseudo seed formation ranged from 34.55% for BC₁F₂ to 63.77 for BC₁F₁ crosses, the haploid embryo formation ranges from 9.43% for BC₁F₁ to 30.2% for BC₁F₂, the haploid plant generation ranges from 19.36% for BC₁F₂ to 63.25% for BC₁F₁. Four doubled haploids were developed from ITSN 105/58 × VL 802 × VL 802 of BC₂F₃ underwent molecular cytogenetic analyses using the probes, viz., rye genomic rDNA, pSc 119 and pAs1. FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

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Keywords

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