Open Access
ARTICLE
Synthesis and characterization of polymeric responsive CMC/Pectin hydrogel films loaded with Tamarix aphylla extract as potential wound dressings
BARKAT ALI KHAN1, FAZAL KARIM1, MUHAMMAD KHALID KHAN1,*, FAHEEM HAIDER1, SADIQULLAH KHAN2
1 Drug Delivery and Cosmetics Laboratory, Gomal Center of Pharmaceutical Sciences, Faculty of Pharmacy, Gomal University, D.I. Khan, 29050, Pakistan
2 Department of Environmental Science, Faculty of Engineering & Technology, Gomal University, D.I. Khan, 29050, Pakistan
* Address correspondence to: Muhammad Khalid Khan, .
(This article belongs to this Special Issue: Biotherapeutic Agents and Innovative Theranostic Formulations: Physical-Chemical Aspects, Pharmacological and Biological Effects)
BIOCELL 2021, 45(5), 1273-1285. https://doi.org/10.32604/biocell.2021.015323
Received 09 December 2020; Accepted 03 February 2021; Issue published 12 July 2021
Abstract
The fourth most predominant overwhelming type of trauma is burn injuries worldwide. Ideal wound healing
dressings help in the wound healing process in a lower time with less pain. Commonly used dry wound dressing, like
absorbent gauze or absorbent cotton, possess limited therapeutic effects and require repeated use, which further
exaggerates patients’ suffering. In contrast, hydrogels films present a promising alternative to improve healing by
guaranteeing a moisture balance at the wound site. The aim of the current study was to synthesize
Tamarix aphylla
(
T. aphylla) extract-loaded hydrogel film with Na-CMC and pectin and to study their wound healing properties. The
Na-CMC/Pectin hydrogels films were synthesized and characterized for HPLC analysis, FTIR, surface morphology,
rheology, tensile strength, swelling behavior, drug release kinetics, and
in vivo wound healing in an animal model.
FTIR confirmed the existence of strong interaction between both polymers but no interaction with the extract. SEM
photographs showed successful embedding of extract in small pores of hydrogel film and showed smooth and
homogenous morphology. Rheological and texture profiles indicated that hydrogels behaved as strong gels. Swelling
and erosion were dependent on the amount of the CMC. HPLC showed drug content of three selected formulation
(A3, E3 and S3) as 85 ± 0.1%, 82.5 ± 0.4% and 80 ± 0.3%, respectively. The release of the drug from the hydrogel was
controlled by a Fickian diffusion mechanism.
In vivo wound healing activity of hydrogel film confirmed that
T. aphylla extract successfully promoted healing rate by significantly reducing (
P < 0.05) the size of wound closure
compared to the control group, evidenced by intensive collagen formation in histopathological and biochemical
analysis. The capability of these hydrogels for burn wounds could be valuable for medical uses as a new window of
safe and effective medication.
Keywords
Cite This Article
KHAN, B. A., KARIM, F., KHAN, M. K., HAIDER, F., KHAN, S. (2021). Synthesis and characterization of polymeric responsive CMC/Pectin hydrogel films loaded with
Tamarix aphylla extract as potential wound dressings.
BIOCELL, 45(5), 1273–1285. https://doi.org/10.32604/biocell.2021.015323
Citations