Open Access

ARTICLE

Isolation, purification and structure elucidation of three new bioactive secondary metabolites from Streptomyces lividans AM

MOHAMMAD EL-METWALLY^1,*, MAMDOUH ABDEL-MOGIB², MANAL ELFEDAWY², GAAD SOHSAH², AHMED REZK³, MAHMOUD MOUSTAFA⁴, MOHAMED SHAABAN^5,6

1 Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, 22511, Egypt
2 Chemistry Department, Faculty of Science, Mansoura University, El-Mansoura, 35516, Egypt
3 Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, 21934, Egypt
4 Biology Department, College of Science, King Khalid University, Abha, 9004, Saudi Arabia
5 Chemistry of Natural Compounds Department, Division of Pharmaceutical Industries, National Research Centre, Dokki-Cairo, 12622, Egypt
6 Organic and Bioorganic Chemistry, Department of Chemistry, Bielefeld University, Bielefeld, 33615, Germany

* Address correspondence to: Mohammad El-Metwally,

BIOCELL 2021, 45(5), 1263-1271. https://doi.org/10.32604/biocell.2021.013198

Received 29 July 2020; Accepted 03 November 2020; Issue published 12 July 2021

Abstract

Microorganisms are a huge mine of bioactive metabolites, and actinomycetes are one of the very active groups in this area. In this article, we are concerned about the full taxonomical characterization of Streptomyces lividans AM, isolated from Egyptian soil. This isolate produced three new bioactive metabolites, namely: 1-Nona-decanoyl,4-oleyl disuccinate (1), filoboletic acid; (9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid (2), and sitosteryl-3β-D-glucoside (3). Extensive 1D and 2D NMR and HR-mass spectrometry were used to elucidate the structures of the three compounds. Moreover, ten known compounds were also identified. The antimicrobial activity of the producing organism and newly reported compounds (1–3) was investigated against a selected group of pathogenic microorganisms. A full taxonomical characterization of the strain was described as well.

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