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ARTICLE
Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata
QICHAO WANG1, WUJING ZENG1, BASHARAT ALI2, XUEMIN ZHANG3, LING XU1,*, ZONGSUO LIANG1,*
1 Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China
2 Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan
3 Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin, 300410, China
* Address correspondence to: Ling Xu, ; Zongsuo Liang,
BIOCELL 2021, 45(4), 1107-1119. https://doi.org/10.32604/biocell.2021.015282
Received 06 December 2020; Accepted 18 January 2021; Issue published 22 April 2021
Abstract
Andrographis paniculata (
A. paniculata) is a Chinese herbal medicine that clears away heat, reduces
inflammation, protects the liver, and promotes choleretics. The WRKYs of
A. paniculata are still not well
characterized, although many WRKYs have been identified in various plant species. In the present study, 59
A.
paniculata WRKY (
ApWRKY) genes were identified and renamed on the basis of their respective chromosome
distribution. These
ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY
domains and combined with WRKY of Arabidopsis. The 59 identified
ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of
A. paniculata. From the structural analysis of the conserved motifs,
different
ApWRKYs structures showed different biological functions, and the
ApWRKY transcription factor had
certain species-specificity in the evolutionary process. The expression patterns of the 41
ApWRKYs were examined
through quantitative real-time PCR (qRT-PCR) in various tissues and under abiotic stresses (salt). The results showed
that most of the
ApWRKY had different reactions to salt treatment. In addition, the content of the four main
secondary metabolites in
A. paniculata leaves was determined under salt stress. The results show that under a low
concentration of salt treatment, the synthesis of andrographolide can be improved.
Keywords
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Cite This Article
APA Style
WANG, Q., ZENG, W., ALI, B., ZHANG, X., XU, L. et al. (2021). Genome-wide identification of wrky gene family and expression analysis under abiotic stresses in andrographis paniculata. BIOCELL, 45(4), 1107-1119. https://doi.org/10.32604/biocell.2021.015282
Vancouver Style
WANG Q, ZENG W, ALI B, ZHANG X, XU L, LIANG Z. Genome-wide identification of wrky gene family and expression analysis under abiotic stresses in andrographis paniculata. BIOCELL . 2021;45(4):1107-1119 https://doi.org/10.32604/biocell.2021.015282
IEEE Style
Q. WANG, W. ZENG, B. ALI, X. ZHANG, L. XU, and Z. LIANG "Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata," BIOCELL , vol. 45, no. 4, pp. 1107-1119. 2021. https://doi.org/10.32604/biocell.2021.015282