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ARTICLE
Angelica sinensis polysaccharides ameliorate 5-flourouracil-induced bone marrow stromal cell proliferation inhibition via regulating Wnt/β-catenin signaling
HANXIANZHI XIAO, RONGJIA QI, ZILING WANG, MINGHE XIAO, YUE XIANG, YAPING WANG, LU WANG*
Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, 40012, China
* Address correspondence to: Lu Wang,
BIOCELL 2021, 45(4), 1045-1058. https://doi.org/10.32604/biocell.2021.015039
Received 17 November 2020; Accepted 11 January 2021; Issue published 22 April 2021
Abstract
Chemotherapy may cause cellular oxidative stress to bone marrow. Oxidative damage of bone marrow
hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment.
Angelica sinensis polysaccharides (ASP) are major effective ingredients of traditional Chinese medicine
Angelica with
multi-target anti-oxidative stress features. In the current study, we investigated the protective roles and mechanisms
of ASP on chemotherapy-induced bone marrow stromal cell (BMSC) damage. The human bone marrow stromal cell
line HS-5 cells were divided into control group, 5-FU group, 5-FU + ASP group, and 5-FU + LiCl group to
investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation inhibition. The results showed that
5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner; however, ASP partially counteracted
the 5-FU-induced decrease in cell viability, whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on
HS-5 cells. ASP reversed the decrease in total cytoplasmic β-catenin, p-GSK-3β, and CyclinD1 following 5-FU
treatment and modulated nuclear expression of β-catenin, Lef-1, and C-myc proteins. Furthermore, ASP also
enhanced the antioxidant capacity of cells and reduced 5-FU-induced oxidative stress, attenuated FoxO1 expression,
thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27
Kip1 expression to
alleviate 5-FU-induced apoptosis and to promote cell cycle progression. All the results above suggest that the
protective role of ASP in 5-FU-treated BMSCs proliferation for the chemotherapy may be related to its activating
Wnt/β-catenin signaling and keeping homeostasis between β-catenin and FoxO1 under oxidative stress. The study
provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.
Keywords
Cite This Article
XIAO, H., QI, R., WANG, Z., XIAO, M., XIANG, Y. et al. (2021).
Angelica sinensis polysaccharides ameliorate 5-flourouracil-induced bone marrow stromal cell proliferation inhibition via regulating Wnt/β-catenin signaling.
BIOCELL, 45(4), 1045–1058. https://doi.org/10.32604/biocell.2021.015039
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