Open Access
ARTICLE
MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2
JUNQI GUO1,2,#, YUN YANG1,2,#, WEI ZHAO1,2, ZHONGHAI YAN3, XIA YANG4, YUNFEI YAN1,2, RUIMIN HAO1,2, JINXIA HU1,2,*, FEI JIAO1,2,*
1 Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, 264003, China
2 Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Yantai, 264003, China
3 Department of Neurology, Columbia University, New York, 10032, USA
4 Department of Cardiology, The People’s Hospital of Zhaoyuan City, Yantai, 265400, China
* Address correspondence to: Jinxia Hu, ; Fei Jiao,
# These authors contributed equally to this work.
BIOCELL 2021, 45(3), 627-638. https://doi.org/10.32604/biocell.2021.013496
Received 08 August 2020; Accepted 25 September 2020; Issue published 03 March 2021
Abstract
Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer
progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer
(NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly
in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16-
5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced
TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between
miR-16-5p and Fermitin family member 2 (FERMT2) was observed, implying there may be a potential link about
their regulation. The hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter assay.
Moreover, we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of
NSCLC cells remarkably accomplished by the expression variance of FERMT2
in vitro and
in vivo assays. Summarily,
this study demonstrated that miR-16-5p, as a tumor suppression factor in NSCLC by targeting FERMT2, could serve
as one promising biomarker in the prediction for NSCLC patients.
Keywords
Cite This Article
GUO, J., YANG, Y., ZHAO, W., YAN, Z., YANG, X. et al. (2021). MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2.
BIOCELL, 45(3), 627–638. https://doi.org/10.32604/biocell.2021.013496