Open Access

ARTICLE

Addition of peroxiredoxin 6 (PRDX6) to IVF fertilization medium maintains motility and longevity of human spermatozoa

by Tiecheng Sun^1,#, Yandong Zhang^1,#, Jianhua Li^3,#, Hong Yu¹, Lingli Song¹, Xinping Sun¹, Xi Chen^2,*, Li Tian^1,2,*

1 Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing, 102206, China
2 Reproductive Medicine Centre, Peking University Second Affiliated Hospital, Beijing, 100044, China
3 Center of Reproductive Medicine and Genetics, Seventh Medical Center of PLA General Hospital, Beijing, 100027, China

* Address correspondence to: Xi Chen, ; Li Tian,
# These authors contributed equally

BIOCELL 2021, 45(3), 705-710. https://doi.org/10.32604/biocell.2021.014694

Received 20 October 2020; Accepted 23 December 2020; Issue published 03 March 2021

Abstract

This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to the control group. The difference in total motility and progressive motility between the 0 and 10−7 mM groups was observed at 24 and 48 h of culture at room temperature. At 24 h, the total motility increased by 30% in the control group (16.03 ± 11.91 vs. 11.51 ± 7.84), and progressive motility increased by 21% (10.53 ± 9.4 vs. 8.31 ± 6.04). A similar trend was observed in the 48 h group. In addition, we also found that peroxiredoxin 6 had a well protective effect on sperm kinetic parameters at 10⁻⁷ mM. The findings of this study suggest that peroxiredoxin 6 can enhance sperm total motility and progressive motility in IVF fertilization medium. Peroxiredoxin 6 may have potential benefits for sperm preparation in assisted reproductive technology.

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Keywords

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