TY - EJOU
AU - HU, ZHAOI
AU - DING, XIANGJUN
AU - JI, YUYAO
AU - LIU, XIAOHONG
AU - DING, ZHIWEN
TI - APEX1 protects against oxidative damage-induced cardiomyocyte apoptosis
T2 - BIOCELL
PY - 2021
VL - 45
IS - 3
SN - 1667-5746
AB - Apurine/pyrimidine-free endonuclease 1 (APEX1) is a multifunctional enzyme that contributes to oxidizationmediated DNA-cleaved base excision repair and redox activation of transcription factors. However, the role of APEX1
during cardiomyocyte oxidative stress injury is not completely understood. In the present study, whether
APEX1 protects oxidative damage-induced cardiomyocytes was investigated. mRNA and protein expression levels of
APEX1 were downregulated in the mouse model of cardiac ischemia-reperfusion injury. Furthermore, the expression
of APEX1 in hydrogen peroxide (H2O2)-treated neonatal mice cardiomyocytes was also decreased. APEX1 knockdown
aggravated H2O2-treated cardiomyocyte apoptosis indexes. By contrast, APEX1 overexpression reversed H2O2-induced
oxidative damage, as demonstrated by decreased caspase 3 and Bax expression levels. Moreover, homeobox A5
upregulated APEX1. The results of the present study indicated that APEX1 displayed protective effects against oxidative
damage, suggesting that APEX1 may serve as a unique protective strategy for cardiac ischemia-reperfusion injury.
KW - Apurine/pyrimidine-free endonuclease 1
KW - Cardiomyocyte apoptosis
KW - Cardiac ischemia-reperfusion
DO - 10.32604/biocell.2021.013293