Open Access

ARTICLE

Acetyl-L-carnitine protects adipose-derived stem cells exposed to H₂O₂ through regulating AMBRA1-related autophagy

by Yao Qian², Jingping Wang², Ziwan Ji², Hao Chen², Yucang He², Xuling Lv², Zikai Zhang², Tian Li², Tianyun Pan³, Liqun Li², Ming Lin^1,*

1 The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325000, China
2 The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China
3 Huzhou Hospital of Traditional Chinese Medicine, Huzhou, 313000, China

* Address correspondence to: Ming Lin,

BIOCELL 2021, 45(1), 189-198. https://doi.org/10.32604/biocell.2021.011827

Received 31 May 2020; Accepted 24 August 2020; Issue published 26 January 2021

Abstract

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H₂O₂ (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.

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