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ARTICLE

Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, Talaromyces purpureogenus KJ584844

SALLY NEGM^1,2, MOHAMMAD EL-METWALLY³, WESAM ELDIN SABER⁴, SAHAR ABO-NEIMA⁵, MAHMOUD MOUSTAFA^6,7,*, ATTALLA EL-KOTT^6,8

1 Life Sciences Department, College of Science and literature Mahyel Aseer, King Khalid University, Abha, Saudi Arabia
2 Unit of Food Bacteriology, Central Laboratory of Food Hygiene, Ministry of Health, Sharkia, Egypt
3 Department of Botany and Microbiology, Faculty of Science, Damanhour University, El-Behera, Egypt
4 Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt
5 Physics Department, Faculty of Science, Damanhour University, Damanhour, Egypt
6 Department of Biology, College of Science, King Khalid University, 9004, Abha, Saudi Arabia
7 Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena, Egypt
8 Zoology Department, Faculty of Science, Damanhour University, El-Behera, Egypt

* Address correspondence to: Mahmoud MOUSTAFA,

(This article belongs to the Special Issue: New Insights in Biology of Depression: New Molecules and Approaches)

BIOCELL 2021, 45(1), 119-127. https://doi.org/10.32604/biocell.2021.012011

Received 10 June 2020; Accepted 22 August 2020; Issue published 26 January 2021

Abstract

The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial fermentation, nanoparticles can play a vital role in this respect. The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process. The interface amongst nitrogen sources (NH₄Cl and yeast extract) was investigated, applying the statistical modeling of central composite design under solid-state fermentation. The optimum medium for proteinase secretion was stimulated by 979.82 mg NH₄Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers. Using Plackett-Burman experimental design, the nanoparticles Co, Ni and Fe were assessed as inducers for proteinase stimulants. Co nanoparticles (5 ppm) were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture, liberating the extreme quantity of amino acids, compared with the lack of the nanoparticles. The findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase catalyst. This approach could be extended to modulate the productivity and activity of similar biomolecules.

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