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Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization

QI SONG1, JUN ZHANG1, QIANG ZHANG1, JING LIU1, KE LV1, JIALU YAO1,2,3,*, YAFENG ZHOU2,3,*

1 Department of Cardiology, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, 215000, China
2 Department of Cardiology, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou, 215000, China
3 Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

* Address correspondence to: Jialu Yao, email; Yafeng Zhou, email

BIOCELL 2020, 44(4), 623-629. https://doi.org/10.32604/biocell.2020.012645

Abstract

Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron microscopy (TEM), nanoparticle-tracking analysis, and western blotting analysis. The expression level of circBCRC-3 in MSCexos was detected by RT-PCR. Finally, the polarization of the RAW264.7 cell phenotype was analyzed by flow cytometry. Moreover, we first identified circBCRC-3 in MSCs. The results further confirmed that MSCexo could effectively shift the macrophage polarization state from M1 towards the M2 phenotype, which indicated its role in MIRI cure.

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APA Style
SONG, Q., ZHANG, J., ZHANG, Q., LIU, J., LV, K. et al. (2020). Exosomes derived from circbcrc-3-knockdown mesenchymal stem cells promoted macrophage polarization. BIOCELL, 44(4), 623-629. https://doi.org/10.32604/biocell.2020.012645
Vancouver Style
SONG Q, ZHANG J, ZHANG Q, LIU J, LV K, YAO J, et al. Exosomes derived from circbcrc-3-knockdown mesenchymal stem cells promoted macrophage polarization. BIOCELL . 2020;44(4):623-629 https://doi.org/10.32604/biocell.2020.012645
IEEE Style
Q. SONG et al., “Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization,” BIOCELL , vol. 44, no. 4, pp. 623-629, 2020. https://doi.org/10.32604/biocell.2020.012645



cc Copyright © 2020 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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