Table of Content

Open AccessOpen Access

ARTICLE

Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization

QI SONG1, JUN ZHANG1, QIANG ZHANG1, JING LIU1, KE LV1, JIALU YAO1,2,3,*, YAFENG ZHOU2,3,*

1 Department of Cardiology, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, 215000, China
2 Department of Cardiology, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou, 215000, China
3 Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

* Address correspondence to: Jialu Yao, ; Yafeng Zhou,

BIOCELL 2020, 44(4), 623-629. https://doi.org/10.32604/biocell.2020.012645

Abstract

Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron microscopy (TEM), nanoparticle-tracking analysis, and western blotting analysis. The expression level of circBCRC-3 in MSCexos was detected by RT-PCR. Finally, the polarization of the RAW264.7 cell phenotype was analyzed by flow cytometry. Moreover, we first identified circBCRC-3 in MSCs. The results further confirmed that MSCexo could effectively shift the macrophage polarization state from M1 towards the M2 phenotype, which indicated its role in MIRI cure.

Keywords


Cite This Article

SONG, Q., ZHANG, J., ZHANG, Q., LIU, J., LV, K. et al. (2020). Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization. BIOCELL, 44(4), 623–629.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1589

    View

  • 1191

    Download

  • 0

    Like

Share Link

WeChat scan