Open Access
ARTICLE
Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization
QI SONG1, JUN ZHANG1, QIANG ZHANG1, JING LIU1, KE LV1, JIALU YAO1,2,3,*, YAFENG ZHOU2,3,*
1 Department of Cardiology, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, 215000, China
2 Department of Cardiology, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou, 215000, China
3 Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China
* Address correspondence to: Jialu Yao, ; Yafeng Zhou,
BIOCELL 2020, 44(4), 623-629. https://doi.org/10.32604/biocell.2020.012645
Received 08 July 2020; Accepted 25 September 2020; Issue published 24 December 2020
Abstract
Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage
shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that
miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly
discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in
MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in
mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron
microscopy (TEM), nanoparticle-tracking analysis, and western blotting analysis. The expression level of circBCRC-3
in MSCexos was detected by RT-PCR. Finally, the polarization of the RAW264.7 cell phenotype was analyzed by flow
cytometry. Moreover, we first identified circBCRC-3 in MSCs. The results further confirmed that MSCexo could
effectively shift the macrophage polarization state from M1 towards the M2 phenotype, which indicated its role
in MIRI cure.
Keywords
Cite This Article
SONG, Q., ZHANG, J., ZHANG, Q., LIU, J., LV, K. et al. (2020). Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization.
BIOCELL, 44(4), 623–629. https://doi.org/10.32604/biocell.2020.012645