Open Access

ARTICLE

GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages

by WU LI, JIANGZHE SI, SHUAI QIU, JING LUO, HUIQIONG SUN, XIAOQING LI, ZHIBIN WAN, WEI GAO, HANLU ZOU, LEI ZHANG, XIAOHONG XIANG, YANZHANG LI, TIESHAN TENG

1 Key Laboratory of Regional Characteristic Agricultural Resources, College of Life Sciences, Neijiang Normal University, Neijiang, 641100, China
2 School of Medical Sciences, College of Medicine, Henan University, Kaifeng, 475004, China
3 School of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing, 401331, China

* Address correspondence to: Tieshan Teng,

(This article belongs to the Special Issue: Bacteriophage Biology and Biotechnology)

BIOCELL 2020, 44(4), 695-701. https://doi.org/10.32604/biocell.2020.011941

Received 06 June 2020; Accepted 12 August 2020; Issue published 24 December 2020

Abstract

The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy. This old approach, which was abandoned in the west in the 1940s but is generating renewed interest, has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts. GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function. In this study, we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis. Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M. smegmatis inside the THP-1 macrophages, which may be linked to the altered lipid profile of the recombinant bacterial cell wall. In a word, this study suggested that gp30, a novel gene from a mycobacteriophage, modulated lipid composition and content to hamper the survivability of bacteria under stress conditions.

---

Keywords

---