Table of Content

Open Access iconOpen Access

ARTICLE

crossmark

Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer

by JUN ZHANG, YEHONG HUANG, WENZHUO LIU, LULU LI, LIMING CHEN

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Department of Biochemistry, School of Life Sciences, Nanjing Normal University, Nanjing, 210023, China

* Address correspondence to: Liming Chen, email

BIOCELL 2020, 44(4), 591-595. https://doi.org/10.32604/biocell.2020.011642

Abstract

Estrogen receptor alpha (ERα/ESR1) is overexpressed in over half of all breast cancers and is considered a valuable therapeutic target in ERα positive breast cancer. Here, we designed a membrane-permeant Chaperonemediated Autophagy Targeting Chimeras (CMATAC) peptide to knockdown endogenous ERα protein through chaperone-mediated autophagy. The peptide contains a cell membrane-penetrating peptide (TAT) that allows the peptide to by-pass the plasma membrane, an αI peptide as a protein-binding peptide (PBD) that binds specifically to ERα, and CMA-targeting peptide (CTM) that targeting chaperone-mediated autophagy. We validated that ERα targeting peptide was able to target and degrade ERα to reduce the viability of ERα positive breast cancer cells. Taken together, our studies provided a new method to reduce the level of intracellular ERα protein via CMATAC, and thus may provide a new strategy for the treatment of ERα positive breast cancer.

Keywords


Cite This Article

APA Style
ZHANG, J., HUANG, Y., LIU, W., LI, L., CHEN, L. (2020). Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer. BIOCELL, 44(4), 591-595. https://doi.org/10.32604/biocell.2020.011642
Vancouver Style
ZHANG J, HUANG Y, LIU W, LI L, CHEN L. Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer. BIOCELL . 2020;44(4):591-595 https://doi.org/10.32604/biocell.2020.011642
IEEE Style
J. ZHANG, Y. HUANG, W. LIU, L. LI, and L. CHEN, “Chaperone-mediated autophagy targeting chimeras (CMATAC) for the degradation of ERα in breast cancer,” BIOCELL , vol. 44, no. 4, pp. 591-595, 2020. https://doi.org/10.32604/biocell.2020.011642

Citations




cc Copyright © 2020 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 3205

    View

  • 1778

    Download

  • 0

    Like

Share Link