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Phytohormonal and metabolism analysis of Brassica rapa L. ssp. pekinensis with different resistance during Plasmodiophora brassicae infection
MEI LAN#, JINGFENG HU#, HONGLI YANG, LIQIN ZHANG, XUEZHONG XU, JIANGMING HE*
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming, 650205, China
* Address correspondence to: Jiangming He,
# Co-first authors
BIOCELL 2020, 44(4), 751-767. https://doi.org/10.32604/biocell.2020.012954
Received 19 July 2020; Accepted 23 September 2020; Issue published 24 December 2020
Abstract
Clubroot of Chinese cabbage (
Brassica rapa L. ssp.
pekinensis), caused by the obligate parasite
Plasmodiophora
brassicae, accounts for serious yield losses. The aim of our study was to explore the phytohormone levels and metabolome
changes in the roots of resistant and susceptible
B. rapa genotypes at a late stage of infection, i.e., 28 days post-infection.
Both genotypes showed decreased auxin levels after
P. brassicae infection except for indole-3-acetic acid. Overall, the
susceptible genotype had higher auxin and cytokinin levels after infection, with the exception of trans-zeatin and 3-
indolebutyric acid as compared to the resistant genotype. Jasmonic acid levels declined after infection regardless of the
genotype. Resistance against clubroot was evident with the increased levels of salicylic acid in the resistant genotype.
The susceptible genotype had a higher number of differentially accumulated metabolites (DAMs) (262) than the
resistant genotype (238) after infection. Interestingly, 132 DAMs were commonly detected in both genotypes when
infected with the pathogen, belonging to metabolite classes such as phenolic acids, amino acids, and derivatives,
glucosinolates, organic acids, flavonoids, nucleotides and derivatives, and fatty acids. The differential metabolite
analysis revealed that metabolites related to amino acid biosynthesis, fatty acid biosynthesis and elongation,
glutathione metabolism, and glucosinolate metabolism were highly accumulated in the resistant genotype, suggesting
their essential roles in resistance against
P. brassicae infection.
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Cite This Article
APA Style
LAN, M., HU, J., YANG, H., ZHANG, L., XU, X. et al. (2020). Phytohormonal and metabolism analysis of brassica rapa L. ssp. pekinensis with different resistance during plasmodiophora brassicae infection. BIOCELL, 44(4), 751-767. https://doi.org/10.32604/biocell.2020.012954
Vancouver Style
LAN M, HU J, YANG H, ZHANG L, XU X, HE J. Phytohormonal and metabolism analysis of brassica rapa L. ssp. pekinensis with different resistance during plasmodiophora brassicae infection. BIOCELL . 2020;44(4):751-767 https://doi.org/10.32604/biocell.2020.012954
IEEE Style
M. LAN, J. HU, H. YANG, L. ZHANG, X. XU, and J. HE "Phytohormonal and metabolism analysis of Brassica rapa L. ssp. pekinensis with different resistance during Plasmodiophora brassicae infection," BIOCELL , vol. 44, no. 4, pp. 751-767. 2020. https://doi.org/10.32604/biocell.2020.012954