Open Access

ARTICLE

Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Phragmites australis

by ZHIHUI WANG, YE YUAN

1 College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319, China
2 Mudanjiang Tobacco Science Research Institute, Haerbin, 150000, China

* Address correspondence to: Ye Yuan,

(This article belongs to the Special Issue: )

BIOCELL 2020, 44(4), 731-736. https://doi.org/10.32604/biocell.2020.010575

Received 11 March 2020; Accepted 26 June 2020; Issue published 24 December 2020

Abstract

Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5- Enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway in microorganisms and plants. The full-length epsps cDNA sequence (paepsps, Genebank: KY860582.1) was cloned and characterized for the first time from Phragmites australis. The full-length cDNA of paepsps was 1308 bp encoding a polypeptide of 435 amino acids. The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants. RT-PCR analysis of paepsps expression indicated that the gene expressed in leaves, stems, and roots, with higher expression in leaves. The expression of the paepsps gene increased with glyphosate application. In addition, the transgenic tobacco containing the paepsps gene showed glyphosate resistance in comparison with control. The novel paepsps is a good candidate gene in transgenic crops with glyphosate tolerance in the future.

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