Open Access

ARTICLE

Optimization of the in-situ growth conditions based on a novel photo-microcalorimeter for the sustainable cultivation of photosynthetic microorganisms

YANJUAN LIAO^1,#, DONGLING YU^2,#, XIAOFANG QIN¹, JINMEI WU¹, HUI WEI¹, HUI LI¹, XIANGYING MA^1,*, ZAIYIN HUANG^1,*

1 Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi University for Nationalities, Nanning, 530006, China
2 The Teaching and Research Department of Biotechnology, Nanning University, Nanning, 530200, China

* Address correspondence to: Xiangying Ma, ; Zaiyin Huang,
# These authors contributed equally to this work

BIOCELL 2020, 44(4), 703-711. https://doi.org/10.32604/biocell.2020.012047

Received 12 June 2020; Accepted 22 September 2020; Issue published 24 December 2020

Abstract

Despite the great potential of photosynthetic microbes in the production of renewable fuels, value-adding chemicals, and water treatment, etc., commercial utilization of them is significantly hindered by the lack of techniques to accurately monitor the thermodynamic and kinetic characteristics of the In-situ growth of microbes under controlled light illumination for optimal cultivation. Herein, we demonstrated that a newly developed highly sensitive photo-microcalorimetric system successfully captured the impacts of the light wavelength and strength on the thermodynamic and kinetic parameters of the In-situ growth of Rhodopseudomonas palustris, a representative photosynthetic microorganism. To our best knowledge, this is the first time that highly precise microcalorimetry is employed to monitor exam the in-situ growth of photosynthetic microorganisms under controllable photo illumination. We envision this technique can help for the optimization of the growth conditions of photosynthetic microorganisms for scale-up applications.

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Keywords

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