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ARTICLE

Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

YUNZE XUAN^1,#, BIN JIN^1,#, SAYAN DEB DUTTA^5,#, MENGMENG LIU², ZAIXIAN SHEN¹, XIWEN LIU³, YANG KANGJUAN^4,*, LIM KI-TAEK^5,*

1 Department of Stomatology, Affiliated Hospital of Yanbian University, Yanji, 133000, China
2 Department of Immunology and Molecular Microbiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, 08826, South Korea
3 Affiliated Hospital of Yanbian University, Yanji, 133000,
4 Department of Cell Biology and Medical Genetics, Yanbian University Medical College, Yanji, 133000, China
5 Department of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, South Korea

* Address correspondence to: Kangjuan Yang, ; Ki-Taek Lim,
# These authors contributed equally to this work

(This article belongs to the Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)

BIOCELL 2020, 44(3), 389-400. https://doi.org/10.32604/biocell.2020.09170

Received 18 November 2019; Accepted 28 April 2020; Issue published 22 September 2020

Abstract

Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay, Alizarin Red-S, and alkaline phosphatase staining, and real-time PCR. Notably, 300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs, indicating their osteogenic potential. Moreover, a significant enhancement of osteogenesis gene markers and proteins was observed. We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation. These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.

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