Open Access

ARTICLE

Effect of peroxiredoxin 6 on total and progressive motility of human spermatozoa after cryopreservation

TIECHENG SUN^1,#, LUYANG CHENG^2,#, JING MA^3,#, SHANJIE ZHOU¹, YANDONG ZHANG¹, WENDI ZHOU⁴, SHOULONG DENG⁵, SHUSONG WANG^3,*, TIAN LI^1,6,*

1 Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing, 102206, China
2 Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China
3 NHC Key Laboratory of Family Planning and Healthy/Key Laboratory of Reproductive Medicine of Hebei Province, Shijiazhuang, 050051, China
4 Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
5 CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China
6 Reproductive Medicine Centre, Peking University Second Affiliated Hospital, Beijing, 100044, China

* Address correspondence to: Shusong Wang, ; Tian Li,
# These authors contributed equally to this work

BIOCELL 2020, 44(3), 323-327. https://doi.org/10.32604/biocell.2020.011890

Received 03 June 2020; Accepted 06 July 2020; Issue published 22 September 2020

Abstract

Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation. However, freezing and thawing significantly reduces the total and progressive motility of human spermatozoa. In the present study, we explored the effects of peroxiredoxin 6 (PRDX6) on total and progressive motility of human spermatozoa after cryopreservation. Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6 (0 mM, 10⁻⁵ mM, 10⁻⁷ mM, and 10⁻⁹ mM, respectively). Postthaw total and progressive motility of sperms were measured. The results showed that in comparison with 0 mM, the concentrations of 10⁻⁵ mM, 10⁻⁷ mM, and 10⁻⁹ mM of PRDX6 all significantly improved total motility and progressive motility of sperms (p < 0.05). The 10⁻⁷ mM of PRDX6 showed the best performance. In conclusion, the supplementation of PRDX6 helps to maintain the total and progressive motility of human spermatozoa.

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