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ARTICLE

DNA damage-induced by sodium flouride (NaF) and the effect of cholicalciferol

by VEYSEL YÜKSEK, SEMIHA DEDE, AYŞE USTA, SEDAT ÇETIN, MEHMET TAŞPINAR

1 Van Yuzuncu Yil University Özalp Vocational High School, Van, Turkey
2 Department of Biochemistry, Faculty of Veterinary Medicine, VanYuzuncu Yil University, Van, Turkey
3 Department of Chemistry, Faculty of Science, VanYuzuncu Yil University, Van, Turkey
4 Department of Medical Biology, Faculty of Medicine, VanYuzuncu Yil University, Van, Turkey

* Address correspondence to: Veysel Yüksek,

BIOCELL 2020, 44(2), 263-268. https://doi.org/10.32604/biocell.2020.09172

Received 18 November 2019; Accepted 15 April 2020; Issue published 27 May 2020

Abstract

It is known that the high electronegativity of fluorine affects various soft tissues, especially the bone structure in organisms. Of these tissues are the kidneys, which play an important role in the excretion of fluoride from the body. Fluoride affects many cellular mechanisms. One of these effects is DNA damage. Our study aimed to investigate the likely protective effect of cholecalciferol (vitamin D3) on genomic DNA damage-induced NaF depending on concentration and time. The IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D₃ through MTT assay in NRK-52E kidney cells were determined. DNA damage was significantly increased (p < 0.05) compared to the control group in all groups except for vitamin D₃. It was determined that treatment with NaF together with vitamin D₃ decreased the DNA damage compared to NaF treated groups for 3 and 12 h. NaF combined with vitamin D3 was determined statistically to decrease (p < 0.05) DNA damage compared to NaF treated groups for 24 h. As a result, it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased (p < 0.05) the genomic DNA damage, but NaF treatment together with vitamin D₃ decreased the DNA damage in renal cells depending on the time. It was concluded that vitamin D₃ may be useful in preventing DNA damage caused by NaF.

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