Table of Content

Open Access iconOpen Access

ARTICLE

crossmark

MicroRNA-382 inhibits the proliferation of mouse spermatogonia by targeting Kmt5a

YI ZHENG#, PENGFEI ZHANG#, JINSHENG ZHU#, LINGKAI ZHANG, WENXIAN ZENG*

Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China

* Address correspondence to: Wenxian Zeng, email
# These authors contributed equally to this work

BIOCELL 2020, 44(2), 201-207. https://doi.org/10.32604/biocell.2020.08770

Abstract

Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility. Essential to this process are spermatogonia capable of both proliferation and differentiation. Nevertheless, the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood. MicroRNAs (miRNAs) are a category of non-coding small RNAs with regulatory functions by binding to the 3’ untranslated region (UTR) of the target mRNA. Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation, differentiation and apoptosis, but the roles of individual miRNAs in spermatogonial fate determination remain largely elusive. Here, by using a mouse spermatogonial cell line (GC-1), we investigated the role for miRNA-382 in spermatogonial proliferation. We found that pre-miRNA-382 was expressed in spermatogonia. The luciferase reporter assay demonstrated Kmt5a but not Top1 as a target gene of miRNA-382. Overexpression of miRNA-382 by transfecting a miRNA mimic downregulated Kmt5a at both RNA and protein levels, and further reduced the proliferation and viability of spermatogonia. Knockdown of Kmt5a by RNA interference (RNAi) resulted in a uniform phenotype in spermatogonia. We therefore conclude that miRNA-382 inhibits the proliferation of mouse spermatogonia by targeting Kmt5a. Our finding extends the knowledge about the regulatory roles of miRNAs in spermatogonia and lays the groundwork for diagnosis and treatment of male infertility.

Keywords


Cite This Article

APA Style
ZHENG, Y., ZHANG, P., ZHU, J., ZHANG, L., ZENG, W. (2020). Microrna-382 inhibits the proliferation of mouse spermatogonia by targeting kmt5a. BIOCELL, 44(2), 201-207. https://doi.org/10.32604/biocell.2020.08770
Vancouver Style
ZHENG Y, ZHANG P, ZHU J, ZHANG L, ZENG W. Microrna-382 inhibits the proliferation of mouse spermatogonia by targeting kmt5a. BIOCELL . 2020;44(2):201-207 https://doi.org/10.32604/biocell.2020.08770
IEEE Style
Y. ZHENG, P. ZHANG, J. ZHU, L. ZHANG, and W. ZENG, “MicroRNA-382 inhibits the proliferation of mouse spermatogonia by targeting Kmt5a,” BIOCELL , vol. 44, no. 2, pp. 201-207, 2020. https://doi.org/10.32604/biocell.2020.08770

Citations




cc Copyright © 2020 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 2913

    View

  • 2224

    Download

  • 1

    Like

Share Link