Open Access

ARTICLE

Synergistic combination of colistin with imipenem, amikacine or ciprofloxacin against Acinetobacter baumannii and Pseudomonas aeruginosa carbapenem-resistant isolated in Annaba hospital Algeria

SAIDA MELIANI¹, SOUMAYA TOUMI¹, HEYTHEM DJAHOUDI², KHALED DEGHDEGH³, KAMEL AMOURA⁴, ABDELGHANI DJAHOUDI^5,*

1 Department of Biochemistry, Faculty of Sciences, Laboratory of Microbiology, Badji Mokhtar University, Annaba, Algeria
2 Department of Biochemistry, Eco-biology Laboratory for Marine and Coastal Areas, Faculty of Sciences, BP 12 El-Hadjar, Badji Mokhtar University, Annaba, Algeria
3 Faculty of Medicine, Pulmonology Service Badji Mokhtar University, Annaba, Algeria
4 Faculty of Medicine, CHU Annaba, Badji Mokhtar University, Annaba, Algeria
5 Department of Pharmacy, Faculty of Medicine, Laboratory of Microbiology, Badji Mokhtar University, Annaba, BP 205, Algeria

* Address correspondence to: Abdelghani Djahoudi,

BIOCELL 2020, 44(2), 175-182. https://doi.org/10.32604/biocell.2020.09097

Received 11 November 2019; Accepted 16 December 2019; Issue published 27 May 2020

Abstract

Objective: The aim of this study is to detect in vitro the synergetic activity of colistin in combination with imipenem, amikacin or ciprofloxacin, at sub-inhibitory concentrations, against carbapenems-resistant (CR) Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from various wards in Annaba teaching hospital in eastern Algeria.
Materials and Methods: The minimal inhibitory concentrations (MIC) were determined by broth macrodilution (BMD). Carbapenemase encoding genes were screened using polymerase chain reaction (PCR). The activity of colistin in combination with second antibiotic was evaluated by the Checkerboard Technique.
Results: 39 CR P. aeruginosa and 21 CR A. baumanni strains where collected. The MIC values ranging from (0.25 to 4 µg/ml) to colistin, ≥16 µg/ml for imipenem, ≥4 µg/ml to amikacin and ≥8 µg/ml ciprofloxacin. The PCR reveals the presence of the genes bla_OXA23 (n = 12), blaOXA24 (n = 6), blaNDM1 (n = 3) in A. baumannii and blaVIM2 (n = 12) in P. aeruginosa. The combination of colistin with imipenem showed synergistic effect on 57.14% and 46.15% of A. baumannii and P. aeruginosa isolates, respectively. For colistin and amikacin, the synergistic effect is detected in 28.6% of A. baumannii and 30.8% of P. aeruginosa. While colistin and ciprofloxacin showed synergy on 14.29% and 15.38% of A. baumannii and P. aeruginosa isolates, respectively.
Conclusion: CR A. baumannii and P. aeruginosa remain the most prevalent infection agents in patients from high-risk wards at Annaba Hospital. Colistin associated with imipenem or with amikacin at sub-inhibitory concentrations gives very encouraging results allowing better management of infections caused by this type of bacteria.

---

Keywords

---

Citations