Open Access

REVIEW

Fungal assembly of L-asparaginase using solid-state fermentation: a review

SALLY NASER^1,2, WESAMELDIN SABER³, MOHAMMAD EL-METWALLY^4,*, MAHMOUD MOUSTAFA^5,6, ATTALLA EL-KOTT^5,7

1 Life Sciences Department, College of Science and Literature Mahyel Aseer, King Khalid University, Abha, Saudi Arabia
2 Unit of Food Bacteriology, Central Laboratory of Food Hygiene, Ministry of Health, Branch in Zagazig, Sharkia, Egypt
3 Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt
4 Botany and Microbiology Department, Faculty of Science, Damanhour University, El-Behera, Egypt
5 Biology Department, College of Science, King Khalid University, Abha, 9004, Saudi Arabia
6 Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt
7 Zoology Department, Faculty of Science, Damanhour University, El-Behera, Egypt

* Address correspondence to: Mohammad El-Metwally,

BIOCELL 2020, 44(2), 147-155. https://doi.org/10.32604/biocell.2020.09522

Received 25 December 2019; Accepted 21 April 2020; Issue published 27 May 2020

Abstract

Because of its antitumor therapeutic-activity, as well as its application in food industries to improve the quality, L-asparaginase has attracted considerable attention from several investigators. In recent years, fungi have occupied advanced rank among microorganisms in the production process of the enzyme. This review is spotting the light on the advantages of fungal enzyme and its applications in the food industry and medications. The solid-state fermentation was discussed as the wide alternative and most accepted biosynthesis technique. However, some lights were also spotted to the statistical experimental design of the fermentation process, mainly on the methodology of the response surface for L-asparaginase biosynthesis by fungi. Finally, the immobilization of the enzyme and the features of the widely used solid substrates for the maximization of the production process were explored.

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