Open Access
ARTICLE
MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NFkappaB signaling pathway
Xin YANG*, Liqun LU, Li HUANG, Jing HE, Jie LV
Department of Pediatrics, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China
*Address correspondence to: Xin Yang,
BIOCELL 2020, 44(1), 101-110. https://doi.org/10.32604/biocell.2020.08324
Issue published 01 March 2020
Abstract
T-cell acute lymphoblastic leukemia (T-ALL) is a hematological tumor caused by the malignant
transformation of immature T-cell progenitor cells. Emerging studies have stated that microRNAs (miRNAs) may play
key roles in T-ALL progression. This study aimed to investigate the roles of miR-145-3p in T-ALL cell proliferation,
invasion, and apoptosis with the involvement of the nuclear factor-kappaB (NF-κB) signaling pathway. T-ALL Jurkat
cells were harvested, and the expression of miR-145-3p and NF-κB-p65 was measured. Gain- and loss-of-functions of
miR-145-3p and NF-κB-p65 were performed to identify their roles in the biological behaviors of Jurkat cells, including
proliferation, apoptosis, and invasion. Consequently, the current study demonstrated that miR-145-3p was downregulated
while NF-κB-p65 was up-regulated in Jurkat cells. miR-145-3p directly bound to the 3’ untranslated region
of NF-κB-p65. Over-expression of miR-145-3p inhibited Jurkat cell proliferation, invasion, and resistance to apoptosis,
while over-expression of NF-κB-p65 presented opposite trends. Co-transfection of miR-145-3p and NF-κB-p65
promoted the malignant behaviors of Jurkat cells compared to miR-145-3p transfection alone, while it reduced these
behaviors of Jurkat cells compared to NF-κB-p65 transfection alone. Taken together, this study provided evidence that
miR-145-3p could suppress proliferation, invasion, and resistance to the death of T-ALL cells via inactivating the NF-
κB signaling pathway.
Keywords
Cite This Article
APA Style
YANG, X., LU, L., HUANG, L., HE, J., LV, J. (2020). Microrna-145-3p suppresses the malignant behaviors of t-cell acute lymphoblastic leukemia jurkat cells via inhibiting the nfkappab signaling pathway. BIOCELL, 44(1), 101-110. https://doi.org/10.32604/biocell.2020.08324
Vancouver Style
YANG X, LU L, HUANG L, HE J, LV J. Microrna-145-3p suppresses the malignant behaviors of t-cell acute lymphoblastic leukemia jurkat cells via inhibiting the nfkappab signaling pathway. BIOCELL . 2020;44(1):101-110 https://doi.org/10.32604/biocell.2020.08324
IEEE Style
X. YANG, L. LU, L. HUANG, J. HE, and J. LV "MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NFkappaB signaling pathway," BIOCELL , vol. 44, no. 1, pp. 101-110. 2020. https://doi.org/10.32604/biocell.2020.08324