Open Access

ARTICLE

MicroRNA-708 inhibits the proliferation and chemoresistance of pancreatic cancer cells

Wensong LIU¹, Yunjie LU¹, Dong ZHANG¹, Longqing SHI¹, Guangchen ZU¹, Haijiao YAN^2,*, Donglin SUN^1,*

1 Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China
2 Department of Oncology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China

* Address correspondence to: Donglin Sun, ; Haijiao Yan,

BIOCELL 2020, 44(1), 73-80. https://doi.org/10.32604/biocell.2020.08613

Issue published 01 March 2020

Abstract

Pancreatic cancer is one of the most aggressive malignancies with poor prognosis and high mortality. Recent studies showed that microRNAs are dysregulated and involved in the initiation and progression of pancreatic cancer. In this study, we found that miR-708 was significantly downregulated in pancreatic cancer tissues and cell lines. Lentivirus-mediated overexpression of miR-708 could significantly inhibit the proliferation and invasion, while enhanced chemosensitivity to gemcitabine in both Panc-1 and SW1990 cells. Luciferase reporter assay showed that miR-708 bound the 3’-untranslated region of survivin and suppressed the expression of survivin in pancreatic cancer cells. In pancreatic cancer tissues, survivin protein was highly expressed and negatively correlated with miR-708 expression. Furthermore, the restoration of survivin expression could partially antagonize proliferation inhibition and apoptosis induction by miR-708 in pancreatic cancer cells. The Panc-1 cells with overexpression of miR-708 also showed decreased proliferation capability in nude mouse model compared with parental cells. In conclusion, our results suggest that miR-708 inhibits pancreatic cancer and could be a novel potential candidate to treat pancreatic cancer.

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