Open Access
ARTICLE
MicroRNA-708 inhibits the proliferation and chemoresistance of pancreatic cancer cells
Wensong LIU1, Yunjie LU1, Dong ZHANG1, Longqing SHI1, Guangchen ZU1, Haijiao YAN2,*, Donglin SUN1,*
1 Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China
2 Department of Oncology, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China
* Address correspondence to: Donglin Sun, ; Haijiao Yan,
BIOCELL 2020, 44(1), 73-80. https://doi.org/10.32604/biocell.2020.08613
Issue published 01 March 2020
Abstract
Pancreatic cancer is one of the most aggressive malignancies with poor prognosis and high mortality.
Recent studies showed that microRNAs are dysregulated and involved in the initiation and progression of pancreatic
cancer. In this study, we found that miR-708 was significantly downregulated in pancreatic cancer tissues and cell
lines. Lentivirus-mediated overexpression of miR-708 could significantly inhibit the proliferation and invasion, while
enhanced chemosensitivity to gemcitabine in both Panc-1 and SW1990 cells. Luciferase reporter assay showed that
miR-708 bound the 3’-untranslated region of survivin and suppressed the expression of survivin in pancreatic cancer
cells. In pancreatic cancer tissues, survivin protein was highly expressed and negatively correlated with miR-708
expression. Furthermore, the restoration of survivin expression could partially antagonize proliferation inhibition
and apoptosis induction by miR-708 in pancreatic cancer cells. The Panc-1 cells with overexpression of miR-708 also
showed decreased proliferation capability in nude mouse model compared with parental cells. In conclusion, our results
suggest that miR-708 inhibits pancreatic cancer and could be a novel potential candidate to treat pancreatic cancer.
Keywords
Cite This Article
LIU, W., LU, Y., ZHANG, D., SHI, L., ZU, G. et al. (2020). MicroRNA-708 inhibits the proliferation and chemoresistance of pancreatic cancer cells.
BIOCELL, 44(1), 73–80. https://doi.org/10.32604/biocell.2020.08613
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