Open Access
ARTICLE
Isolation and molecular identification of cellulolytic bacteria from Dig Rostam hot spring and study of their cellulase activity
Sareh HAJIABADI1, Mansour MASHREGHI1, Ahmad Reza BAHRAMI1,2, Kiarash GHAZVINI3, Maryam M. MATIN1,2,*
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
3 Microbiology Research Center & Department of Microbiology and Virology, Ghaem Medical Center, Mashhad University of Medical Sciences, Mashhad, Iran
* Address correspondence to: Maryam M. Matin,
BIOCELL 2020, 44(1), 63-71. https://doi.org/10.32604/biocell.2020.08171
Issue published 01 March 2020
Abstract
Cellulose is the main structural component of lignocellulosic wastes that can be converted to sugars and
biofuels by cellulase. Due to wide applications of this enzyme in various industries around the world, cellulase is
considered as the third industrial enzyme. The ability of thermophilic bacteria in the production of heat-stable cellulases
has made them valuable tools in biotechnology. The aim of this study was isolation and molecular identification of
cellulolytic thermophile bacteria from Dig Rostam hot spring and investigating their cellulase activity. Samples were
taken from water and sediments of this hot spring, and cellulolytic bacteria were enriched in media containing cellulose
as the only carbon source. The bacteria were incubated at 60°C, and single colonies were then isolated on solid media.
Congo red assay was used as a quick test for the qualitative screening of cellulase activity. According to these qualitative
results, four colonies named CDB1, CDB2, CDB3, and CDB4 were isolated, and their growth curve and some other
characteristics were determined by biochemical assays. Moreover, endoglucanase, exoglucanase, and FPase activities
of the isolates were investigated quantitatively. Results indicated that CDB1 exhibited the highest endoglucanase (0.096
U/mL) and exoglucanase (0.156 U/mL) activities among other isolates. 16S rDNA partial sequencing indicated that
CDB1 had 99% similarity to the genus
Anoxybacillus, and the other isolates showed the highest similarity to the genus
Geobacillus. The cellulase gene of CDB1 isolate with the highest cellulase activity was also cloned, and its sequence is
reported for the first time. Further studies on this thermophilic enzyme might be useful for industrial applications.
Keywords
Cite This Article
APA Style
HAJIABADI, S., MASHREGHI, M., BAHRAMI, A.R., GHAZVINI, K., MATIN, M.M. (2020). Isolation and molecular identification of cellulolytic bacteria from dig rostam hot spring and study of their cellulase activity. BIOCELL, 44(1), 63-71. https://doi.org/10.32604/biocell.2020.08171
Vancouver Style
HAJIABADI S, MASHREGHI M, BAHRAMI AR, GHAZVINI K, MATIN MM. Isolation and molecular identification of cellulolytic bacteria from dig rostam hot spring and study of their cellulase activity. BIOCELL . 2020;44(1):63-71 https://doi.org/10.32604/biocell.2020.08171
IEEE Style
S. HAJIABADI, M. MASHREGHI, A.R. BAHRAMI, K. GHAZVINI, and M.M. MATIN "Isolation and molecular identification of cellulolytic bacteria from Dig Rostam hot spring and study of their cellulase activity," BIOCELL , vol. 44, no. 1, pp. 63-71. 2020. https://doi.org/10.32604/biocell.2020.08171
Citations